Plant Cell and Tissue Culture, Softcover reprint of the original 1st ed. 1982
A Laboratory Manual

The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu­ are now established in many research laboratories racy with sufficient illustration to make all mani­ throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec­ and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists.

I Introduction.- II Isolation of Plant Material and Studies on Growth and Cell Division.- Experiment 1 Isolation of Explants, Establishment and Maintenance of Callus (Daucus carota).- Sub-Culture of Callus.- Experiment 2 Growth and Cell Division in Cultured Artichoke Explants.- Experiment 3 Initiation and Establishment of Cell Suspension Cultures of Carrot (Daucus carota).- Experiment 4 Isolation and Culture of Single Cells and Examination of the “Conditioning” Effect.- III Bioassay Systems for Cytokinins.- Experiment 5 Soybean Bioassay System.- Experiment 6 Tobacco Callus Bioassay System.- IV Morphogenesis in Vitro: Studies on Regeneration.- Experiment 7 Embryogenesis in Cultured Cells of Carrot.- Experiment 8 The Tobacco Pith System.- Experiment 9 Culture of Anthers from Nicotiana tabacum and the Establishment of Haploid Plants from Embryos.- Experiment 10 Vegetative Propagation of Orchids (Cymbidium).- V Isolation, Culture and Fusion of Protoplasts from Higher Plants.- Experiment 11 Isolation and Culture of Mesophyll Protoplasts from Tobacco Leaves.- Experiment 12 Protoplast Fusion Induced by Polyethylene Glycol (PEG).- VI Secondary Metabolites in Tissue Culture.- Experiment 13 Callus Formation and Anthocyanin Production in Cultures of Haplopappus gracilis.- Experiment 14 The Production of the Steroid, Diosgenin, from Tissue Cultures of Dioscorea deltoidea.- VII Embryo and Organ Culture.- Experiment 15 Embryos of Maize (Zea mays).- Experiment 16 The Isolation and Culture of the Primary Seedling Root of Pea (Pisum sativum).- Experiment 17 Isolation and Culture of the Shoot Apex of Carnation.- VIII Appendix.- IX Index.