Description
Principles and Reactions of Protein Extraction, Purification, and Characterization
Authors: Ahmed Hafiz, Ahmed PhD Hafiz
Language: English
Subjects for Principles and Reactions of Protein Extraction...:
Keywords
Anion Exchange Chromatography; Acrylamide Stock Solution; Protein Purification; Equilibrating Buffer; Mass Spectrometry; Hat Medium; Recombinant Proteins; Stacking Gel; Protein Characterization; IEF; NMR; Destaining Solution; Protein Extraction; IgG Solution; Amplex Red Reagent; Stacking Gel Buffer; Bolton Hunter Reagent; Ammonium Persulfate; Biotin Hydrazide; Gel Cassette; Non-denaturing Page; Lowry Assay; Staining Solution; Primary Antibody; Desalting Column; Gel Buffer; Gel Filtration Chromatography; Fast Red TR; Homobifunctional Cross-Linkers; Eluting Buffer; Methionine Free Medium
Publication date: 07-2017
· 15.6x23.4 cm · Hardback
250.90 €
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the book of Ahmed Hafiz, Ahmed PhD Hafiz
Publication date: 09-2004
388 p. · 15.6x23.4 cm · Spiral
Description
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Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization.
The author presents the principles and reactions behind each procedure and uses tables to compare the different methods. The book also discusses the development of antibodies and immunochemical techniques as tools for characterizing proteins and modified proteins such as glycoproteins. Helpful illustrations, diagrams, and tables effectively transform theoretical concepts into practical knowledge. Along with methodical working procedures for most techniques, the book also offers useful advice on which technique to use and when to apply a particular method.
Presenting the advantages and disadvantages of the various protein techniques, Principles and Reactions of Protein Extraction, Purification, and Characterization enables students and researchers to master the mechanisms behind the protocols and choose the best method for their purposes.
