Description
Protein Acetylation, Softcover reprint of the original 1st ed. 2013
Methods and Protocols
Methods in Molecular Biology Series, Vol. 981
Coordinators: Hake Sandra B, Janzen Christian J
Language: EnglishSubject for Protein Acetylation:
Support: Print on demand
266 p. · 17.8x25.4 cm · Hardback
Description
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Thousands of proteins have been identified to be acetylated. Immense research power has been dedicated to experiments to solve the biological implications of each and every protein acetylation. Two particular sites of protein acetylation have been described intensively: the N-terminal methionine residue of a nascent protein and lysine residues within a protein. In Protein Acetylation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study protein acetylation. These include methods and techniques for identification of protein acetylation, column- and gel electrophoresis-based approaches, computationally prediction, and the biological response to protein acetylation. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Protein Acetylation: Methods and Protocols seeks to aid scientists in the further study of the technical aspects involved in understanding protein acetylation.
1. Validation of Protein Acetylation by Mass Spectrometry
Barry M. Zee and Benjamin A. Garcia
2. Application of the CIRAD Mass Spectrometry Apprach for Lysine Acetylation Site Discovery
Caroline A Evans, Saw Yen Ow, Duncan L Smith, Bernard M Corfe, and Phillip C Wright
3. Application of the MIDAS Approach for Analysis of Lysine Acetylation Sites
Caroline A Evans, John R Griffiths, Richard D Unwin, Anthony D Whetton, Bernard M Corfe
4. Application of High Content Biology to Yield Quantitative Spatial Proteomic Information on Protein Acetylations
Bernard M Corfe, Josephine Kilner, Joanna Chowdry, Roderick SP Benson, Gareth J Griffiths, Caroline A Evans
5. Towards the N-terminal Acetylome: An N-terminal Acetylated Peptide Enrichment Method Using CNBr-Activated Sepharose Resin
Xumin Zhang and Peter Højrup
6. Identification and Analysis of O-acetylated Sialoglycoproteins
Chandan Mandal and Chitra Mandal
7. HPLC-based Quantification of in vitro N-terminal Acetylation
Rune H. Evjenth, Petra Van Damme, Kris Gevaert, Thomas Arnesen
8. Separation and Purification of Multiply Acetylated Proteins using Cation-exchange Chromatography
Romeo Papazyan and Sean D. Taverna
9. In-gel N-acetylation for the Quantification of the Degree of Protein in vivo N-terminal Acetylation
Petra Van Damme, Thomas Arnesen, Bart Ruttens, Kris Gevaert
10. Computational Prediction of Lysine Acetylation Proteome-wide
Amrita Basu
11. Generation and Characterization of Pan-specific Anti-acetyllysine Antibody
Dr. Wei Xu and Dr. Shimin Zhao
12. Using Functional Proteome Microarrarys to Study Protein Lysine Acetylation
Jin-Ying Lu, Yu-yi Lin, Jef D. Boeke, and Heng Zhu
13. Quantitation of Nucleosome Acteylation and other Histone Post-Translational Using Microscale NU-ELISA
Bo Dai, Charles Giardina, Theodore P. Rasmussen
14. Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome Core
John C. Shimko, Cecil J. Howard, Michael G. Poirier, and Jennifer J. Ottesen
15. Production of Amino-terminally Acetylated Recombinant Proteins in E. coli
Matthew Johnson, Michael A. Geeves and Daniel P. Mulvihill
16. Identification of Lysine Acetyltransferase Substrates using Bioorthogonal Chemical Proteomics
Markus Grammel and Howard C. Hang
17. Non-radioactive in-vitro Assays for Histone Deacetylases
Alexander-Thomas Hauser, Julia M. Gajer (née Wagner), and Manfred Jung
18. The Fluorescence-Based Acetylation Assay Using Thiol-Sensitive Probes
Tielong Gao, Chao Yang, Yujun George Zheng
19. Analysis of Protein Acetyltransferase Structure-function Relation by Surface-Enhanced Raman Scattering (SERS): A Tool to Screen and Characterize Small Molecule Modulators
Mohammed Arif, Dhanasekaran Karthigeyan, Soumik Siddhanta, G. V. Pavan Kumar, Chandrabhas Narayana, and Tapas K. Kundu
Features cutting-edge laboratory protocols dealing with protein acetylation
Addresses the functional consequences of protein acetylation
Contains expert tips on troubleshooting and avoiding known pitfalls
Includes supplementary material: sn.pub/extras