Proteomics and Protein-Protein Interactions, 2005
Biology, Chemistry, Bioinformatics, and Drug Design
Protein Reviews Series, Vol. 3

Gabriel Waksman Institute of Structural Molecular Biology, Birkbeck and University College London, Malet Street, London WC1E 7HX, United Kingdom Address for correspondence: Professor Gabriel Waksman Institute of Structural Molecular Biology Birkbeck and University College London Malet Street London WC1E 7H United Kingdom Email: g. waksman@bbk. ac. uk and g. waksman@ucl. ac. uk Phone: (+44) (0) 207 631 6833 Fax: (+44) (0) 207 631 6833 URL: http://people. cryst. bbk. ac. uk/?ubcg54a Gabriel Waksman is Professor of Structural Molecular Biology at the Institute of Structural Molecular Biology at UCL/Birkbeck, of which he is also the director. Before joining the faculty of UCL and Birkbeck, he was the Roy and Diana Vagelos Professor of Biochemistry and Molecular Biophysics at the Washington University School of Medicine in St Louis (USA). The rapidly evolving ?eld of protein science has now come to realize the ubiquity and importance of protein?protein interactions. It had been known for some time that proteins may interact with each other to form functional complexes, but it was thought to be the property of only a handful of key proteins. However, with the advent of hi- throughput proteomics to monitor protein?protein interactions at an organism level, we can now safely state that protein?protein interactions are the norm and not the exception.

Introduction: Proteomics and Protein-Protein Interactions: Biology, Chemistry, Bioinformatics, and Drug Design.- Yeast Two-Hybrid Protein-Protein Interaction Networks.- The Use of Mass Spectrometry in Studying Protein-Protein Interaction.- Molecular Recognition in the Immune System.- Computational Methods for Predicting Protein-Protein Interactions.- Protein-Protein Docking Methods.- Thermochemistry of Binary and Ternary Protein Interactions Measured by Titration Calorimetry: Complex Formation of CD4, HIV gp120, and Anti-gp120.- Protein-Protein Recognition in Phosphotyrosine-Mediated Intracellular Signaling.- Competitive Binding of Proline-Rich Sequences by SH3, WW, and Other Functionally Related Protein Domains.- The Structure and Molecular Interactions of the Bromodomain.- SMART Drug Design: Novel Phosphopeptide and ATP Mimetic-Based Small Molecule Inhibitors of the Oncogenic Protein Kinase pp60src (Src).- Disrupting Protein-Protein Interaction: Therapeutic Tools Against Brain Damage.- A Thermodynamic Guide to Affinity Optimization of Drug Candidates.

The only book that gives such a broad overview of protein-protein interactions and their importance Provides a complete view of the field by addressing issues on protein-protein interactions from their discovery (2 hybrid and mass spectrometry), to their biochemical and biophysical characterization, to their bioinformatics treatment, to their exploration as drug targets In the age of proteomics, i.e. large scale and high throughput mode of discovery, the book not only provides insights into the new techonology, its scope, and its limits, but also connects to the more traditional ways of looking into the issue One theme permeating all chapters in the book is the use of structural biology as a common denominator to study protein-protein interactions; this is unique to this book. Due to the resulting rich color illustrations, the book will be printed in full color throughout Each chapter has been written by leading experts in their respective field Includes supplementary material: sn.pub/extras