Receptor Purification, 1990
Volume 1 Receptors for CNS Agents, Growth Factors, Hormones, and Related Substances
Receptor Purification Series, Vol. 1

The purpose of these volumes is to provide a reference work for the methods of purifying many of the receptors we know about. This be­ comes increasingly important as full-length receptors are overexpressed in bacteria or in insect cell systems. A major problem for abundantly expressed proteins will be their purification. In addition to purification protocols, many other details can be found concerning an individual receptor that may not be available in standard texts or monographs. No book of this type is available as a compendium of purification procedures. Receptor Purification provides protocols for the purification of a wide variety of receptors. These include receptors that bind: neurotransmit­ ters, polypeptide hormones, steroid hormones, and ligands for related members of the steroid supergene family and others, including receptors involved in bacterial motion. The text of this information is substantial, so as to require its publication in two volumes. Consequently, a division was made by grouping receptors by the nature of their ligands. Thus, in Volume One there are contributions on serotonin receptors, adrenergic receptors, the purification of GTP-binding proteins, opioid receptors, neurotensin receptor, luteinizing hormone receptor, human chorionic gonadotropin receptor, follicle stimulating hormone receptor, thyro­ tropin receptor, prolactin receptor, epidermal growth factor receptor, platelet derived growth factor receptor, colony stimulating factor recep­ tor, insulin-like growth factor receptors, insulin receptor, fibronectin receptor, interferon receptor, and the cholecystokinin receptor.

Serotonin Receptors.- Purification and Cloning of Central Serotonin Receptors.- Purification of ? -Adrenergic Receptors: Isolation of Mammalian ?,-and ?2-Subtypes.- Purification of ?, ?Subunits of Guanine Nucleotide Binding Proteins.- Purification of Opioid Binding Proteins.- µ-Opioid Receptor: Purification and Reconstitutionwith GTP-Binding Protein.- The Neurotensin Receptor from Mammalian Brain: Solubilization and Purification by Affinity Chromatography.- The Luteinizing Hormone/Human Chorionic Gonadotropin Receptor of Testis and Ovary: Purification and Characterization.- Purification of LH/hCG Receptor.- The Follicle Stimulating Hormone Receptor.- Thyrotropin Receptor:Characterization and Purification.- Thyrotropin Receptor Purification.- Prolactin Receptors: Purification, Molecular Weight, and Binding Specificity.- Ligand-and Antibody-Affinity Purification of the Epidermal Growth Factor Receptor.- Platelet-Derived Growth Factor B Type Receptor.- Colony-Stimulating Factor 1 Receptor.- Insulin-Like Growth Factor-II Receptors.- Purification of Insulin and Insulin-Like Growth Factor (IGF)-I Receptor.- Insulin Receptor Isolation Through Avidin-Biotin Technology.- Insulin Receptor Purification.- Insulin Receptor Purification.- Isolation of Fibronectin Receptors.- Fibronectin Receptor Purification Using the Complementary Hydropathy Approach.- Purification of the Human Interferon-y Receptor by Ligand Affinity.- Purification of the Cholecystokinin Receptor.