Regulatory T Cells, 2011
Methods and Protocols
Methods in Molecular Biology Series, Vol. 707

Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses. In Regulatory T Cells: Methods and Protocols, experts in the field to offer a collection of current techniques to advance the study of regulatory T cells, including the use of the IL-2 receptor alpha chain and other markers, as well as the more recently desirable use of the transcription factor FoxP3. Divided into three sections, the book covers, in equal measure, in vitro, in vivo, and human studies. Written in the highly successful Methods in Molecular Biology? series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known methodological pitfalls. Authoritative and cutting-edge, Regulatory T Cells: Methods and Protocols distills the most vital current techniques through several years of optimization and standardization in order to allow reliable and reproducible use by both young and experienced cellular and molecular immunologists.

Regulatory T Cells: History and Perspective.- In vitro Treg Suppression Assays.- Generation of T Cell Hybridomas from Naturally-Occurring FoxP3+ Regulatory T Cells.- In vitro and In vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells.- Flow Cytometric Profiling of Mature and Developing Regulatory T Cells in the Thymus.- ChIP-on-Chip for FoxP3.- Live Imaging of Dendritic Cell-Treg Cell Interactions.- Genetic Tools for Analysis of FoxP3+ Regulatory T Cells In vivo.- In vivo Treg Suppression Assays.- In vivo Depletion of FoxP3+ Tregs Using the DEREG Mouse Model.- Antigen-Specific Induction of Regulatory T Cells In vivo and In vitro.- In vitro Expansion of Alloantigen-Specific Regulatory T Cells and Their Use in Prevention of Allograft Rejection.- Analysis of Human FoxP3+ Treg Cells Phenotype and Function.- Depletion of Human Regulatory T Cells.- Assessment of Suppressive Capacity by Human Regulatory T Cells Using a Reproducible, Bi-Directional CFSE-Based In vitro Assay.- Measurement of Proliferation and Disappearance of Regulatory T Cells in Human Studies Using Deuterium-Labeled Glucose.- Flow Cytometric Detection of Human Regulatory T Cells.

Provides optimized and standardized protocols to allow reliable and reproducible use Presents innovations that have lead to insights on the suppressive mechanisms and biology of regulatory T cells Features tips from the experts to ensure the flawless implementation of the techniques Includes supplementary material: sn.pub/extras