Super-Resolution Microscopy, Softcover reprint of the original 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1663

This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Super-Resolution Microscopy:Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.

Preface…
Table of Contents…
Contributing Authors…

1)  Super-Resolution Microscopy Techniques and their Potential for Applications in Radiation Biophysics
Jan Philipp Eberle, Alexander Rapp, Matthias Krufczik, Marion Eryilmaz, Manuel Gunkel, Holger Erfle, and Michael Hausmann

2)      Managing the Introduction of Super-Resolution Microscopy into a Core Facility
Jeffrey A. Kamykowski and Brian Storrie

3)      Live-Cell STED Imaging with the HyPer2 Biosensor
Natalia M. Mishina and Vsevolod V. Belousov

4)      Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope
André Klauss and Carsten Hille

5)      Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo
Mirelle J.T. ter Veer, Thomas Pfeiffer, and U. Valentin Nägerl

6)      STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells
Roman S. Erdmann, Derek Toomre, and Alanna Schepartz

7)      Four Channel Super-Resolution Imaging by 3-D Structured Illumination
Ulrike Engel

8)      Correlative SIM-STORM Microscopy
O. Burri, T. Laroche, R. Guiet, and A. Seitz

9)      Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples
Patricia Bondia, Santiago Casado, and Cristina Flors

10)   Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles
Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, and Mike Heilemann

11)   Two-Color Single-Molecule Tracking in Live Cells
Siegfried Hänselmann and Dirk-Peter Herten

12)   Fully Automated Targeted Confocal and Single Molecule Localization Microscopy
Jan Philipp Eberle , Walter Muranyi , Hans-Georg Kräusslich , Holger Erfle, and Manuel Gunkel

13)   Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy
Christopher L. German, Manasa V. Gudheti, Annette E. Fleckenstein, and Erik M. Jorgensen

14)   Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells
Errin Johnson and Rainer Kaufmann

15)   Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and their use in Cellular Imaging Experiments
Jonathan B. Grimm, Timothy A. Brown, Brian P. English, Timothée Lionnet, and Luke D. Lavis

16)   Measuring Nanometer Distances between Fluorescent Labels Step-by-Step
Susanna Maria Früh and Ingmar Schoen

17)   Correlative Single Molecule Localization Microscopy and Confocal Microscopy
Christian Soeller, Yufeng Hou, Isuru D. Jayasinghe, David Baddeley, and David Crossman

18)   Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells
Kem A. Sochacki and Justin W. Taraska

19)   In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT
Brian J. Beliveau, Alistair N. Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang, and C.-ting Wu

20)   Super-Resolution High Content Screening and Analysis
K. Soliman

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts