Techniques in Glycobiology
Coordonnateurs : Townsend R. Reid, Hotchkiss Arland T.
Mass spectrometry and NMR: new frontiers in nuclear magnetic resonance spectroscopy - use of the Nano.NMR probe for the analysis of microgram quantities of complex carbohydrates; analysis of anionic glycoconjugates by delayed extraction matrix-assisted laser desorption time-of-flight mass spectrometry. Glycolipids and the new physical methods: microscale analysis of glycosphingolipids by thin-layer chromatography blotting; high-performance liquid chromatography of gangliosides; a sensitive cell suspension enzyme-linked immunosorbent assay measuring the density of cell surface ganglioside and carbohydrate antigens. Protein 0-glycosylation: effects of 0-glycosylation on protein structure - the role and characterization of heavily 0-glycosylated mucin domains; predicting mucin-type 0-glycosylation sites; experimental approaches to studying 0-glycosylation in vivo. Labelling oligosaccharides with chromophore and fluorophores: highly sensitive pre-column derivation procedures for quantitative determination of monosaccharides, sialic acids, and amino sugar alcohols of glycoproteins by reversed phase high-performance liquid chromatography; glycan labelling with the fluorophores 2-aminobenzamide and anthranilic acid. Strategies for oligosaccharides separations and sequencing: discovery and uses of novel glucosidases: resolution of oligosaccharides in capillary electrophoresis; a comparison of oligosaccharides profiling methods. Plant glycobiology: recent developments in starch structure analysis using high-performance anion-exchange chromatography with pulsed amperometric detection; red seaweed galactants - methodology for the structural determination of corallinan, a different agaroid. (Part contents)>
Date de parution : 09-2019
15.2x22.9 cm
Thèmes de Techniques in Glycobiology :
Mots-clés :
PTH Amino Acid; BIO-Gel P2; Phenol Sulfuric Acid Assay; Anion Exchange Chromatographies; PVDF; Polyvinylidene Difluoride; High pH Anion Exchange Chromatography; Sialic Acids; Centrifugal Vacuum Evaporator; Laser Desorption Ionization Mass Spectrometry; Pulsed Amperometric Detection; Bovine Fetuin; Electrospray Ionization Mass Spectrometry; Oxford GlycoSystems; Sample Preparation; Liquid Secondary Ionization Mass Spectrometry; Reductive Amination; HPTLC Plate; Glycosidase Activity; Neutral Oligosaccharides; LIF; Phase HPLC; Neutral Sugars; Bovis BCG; DPP Iv