Transcription Factors, Softcover reprint of the original 1st ed. 2010 Methods and Protocols Methods in Molecular Biology Series, Vol. 647
Coordonnateur : Higgins Paul J.
In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription Factors: Methods and Protocols, experts in the field describe state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function. The chapters are written by prominent scientists, many of whom developed these methods, and highlight protocols that focus on specific transcription factor family members with particular relevance to human disease. Composed in the highly successful Methods in Molecular Biology? series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and current, Transcription Factors: Methods and Protocols compiles the latest techniques for elucidating controls on transcription factor intracellular localization and activity, and consequently is unlike any other methods-based text on transcriptional regulation today.
The methods chapters are preceded by several excellent reviews that highlight the role of post-translational modifications and subcellular trafficking in transcription factor function
Includes new techniques for assessing kinase targeting of specific transcription factors, sites of phosphorylation and the involved signaling pathways
Describes novel molecular and elegant microscopic imaging-based procedures to evaluate nuclear translocation and export in living cells
Includes supplementary material: sn.pub/extras
Date de parution : 08-2016
Ouvrage de 426 p.
17.8x25.4 cm
Date de parution : 06-2010
Ouvrage de 426 p.
17.8x25.4 cm