Transgenic Mouse Methods and Protocols (2^nd Ed., 2nd ed. 2011) Methods in Molecular Biology Series, Vol. 693

The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology? series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state of the art, Transgenic Mouse Methods and Protocols, second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.

Table of Contents Preface Contributors 1. Introduction: Strategies for developing genetically modified mice Marten H. Hofker 2. Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst Injection Jan Willem Vonken 3. Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos Jérôme Artus and Anna-Katerina Hadjantonakis 4. Cryopreservation of mouse spermatozoa and In vitro fertilization Naomi Nakagata 5. Autopsy and histological analysis of the transgenic mouse Marion J.J. Gijbels and Menno P.J. de Winther 6. Transgene design Bart van de Sluis and Jan Willem Vonken 7. Inducible Transgenic Mouse Models Thomas L. Saunders 8. Lentiviral transgenesis Terunaga Nakagawa and Casper C. Hoogenraad 9. Transgenesis in Mouse Embryonic Stem Cells Janine van Ree, Wei Zhou, Ming Li and Jan van Deursen 10. Engineering BAC Reporter Gene Constructs for Mouse Transgenesis Yu Fu and Peter Maye 11. Targeting Vector Construction Through Recombineering Liviu A. Malureanu 12. Generating Conditional Knockout Mice Roland H. Friedel, Wolfgang Wurst, Benedikt Wefers and Ralf Kühn 13. Hypomorphic mice Daren J. Baker 14. MICER Targeting Vectors for Manipulating the Mouse Genome Chunhong Liu, Paul F. Szurek and Y. Eugene Yu 15. Knockin approaches Anton J.M. Roebroek, Philip L.S.M. Gordts and Sara Reekmans 16. Generation of a series of knockin alleles using RMCE in ES cells Anton J.M. Roebroek, Philip L.S.M. Gordts and Sara Reekmans 17. Selection of targeted mutants from a library of randomly mutagenized ES cells Kyoji Horie, George Gaitanaris, and Alexander Gragerov 18. Generation of genetically modified rodents using random ENU mutagenesis Ruben van Boxtel and Edwin Cuppen 19. Bone marrow transplantations to study gene function in hematopoietic cells Menno P.J. de Winther and Peter Heeringa 20. Adenovirus Mediated Gene Transfer Ko Willems van Dijk, Kyriakos E. Kypreos, Frits J. Fallaux and Jurre Hageman