Description
Trinucleotide Repeats, 1st ed. 2020
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2056
Language: EnglishPublication date: 10-2020
Support: Print on demand
286 p. · 17.8x25.4 cm · Hardback
Description
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1. Experimenting with Trinucleotide Repeats: Facts and Technical Issues
Guy-Franck Richard
2. Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions
Stéphanie Tomé and Geneviève Gourdon
3. Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae
Gregory M. Williams, Athena K. Petrides, Lata Balakrishnan, and Jennifer A. Surtees
4. Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis
David Viterbo and Guy-Franck Richard
5. Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae
Erica J. Polleys and Catherine H. Freudenreich
6. Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae
Wenying Guo and Kirill S. Lobachev
7. Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay
Lucie Poggi, Bruno Dumas, and Guy-Franck Richard
8. Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site
Rujuta Yashodhan Gadgil, S. Dean Rider, Todd Lewis, Joanna Barthelemy, and Michael Leffak
9. Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells
Artem V. Kononenko, Thomas Ebersole, and Sergei M. Mirkin
10. Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture
Gregory M. Williams and Robert S. Lahue
11. The Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model
Xiaonan Zhao, Huiyan Lu, Pradeep K. Dagur, and Karen Usdin
12. In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA
Remco T.P. van Cruchten and Derick G. Wansink
13. FISH Protocol for Myotonic Dystrophy Type 1 Cells
Arnaud F. Klein, Ludovic Arandel, Joelle Marie, and Denis Furling
14. Real Time Videomicroscopy and Semi-Automated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases
Sandra O. Braz, Diana M. Dinca, Geneviève Gourdon, and Mário Gomes-Pereira
15. Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia
Olivier Griso and Hélène Puccio
16. GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats
Cinzia Cinesi, Bin Yang, and Vincent Dion
17. Gene Therapy for Huntington's Disease Using Targeted Endonucleases
Magdalena Dabrowska and Marta Olejniczak
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
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