Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Softcover reprint of the original 1st ed. 2015
Methods in Molecular Biology Series, Vol. 1247

This volume provides an overview of molecular biology tools for pathogen detection and characterization. Focusing on biobanking, biosafety,  good laboratory practices, biological specimen collection and processing, quality assurance and control, validation of molecular diagnostic assays for veterinary use; molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches; genotyping tools for assessing the genetic landscape and epidemiology of pathogens; and integrative omics and high-throughput technologies as powerful research tools. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies aids scientists in continuing to study a broad range of molecular approaches, from simple and affordable to emerging and sophisticate.

PART I- Introducing Nucleic Acid Testing In The Veterinary Laboratory

1. Overview And Challenges Of Molecular Technologies In The Veterinary Microbiology Laboratory

Mónica V. CunhaAnd João Inácio

2. Significance And Integration Of Molecular Diagnostics in the Framework of Veterinary Practice

Alicia Aranaz

3. Biosafety Principles And Practices for the Veterinary Diagnostic Laboratory

Joseph Kozlovac And Beverly Schmitt

 4. Veterinary Biobank Facility: Development And Management For Diagnostic and Research Purposes

Tina Lombardo, Silvia Dotti, Riccardo Villa, Stefano Cinotti, And Maura Ferrari

 5. Biological Specimen Collection And Processing for Molecular Analysis

Susan SanchezAnd Holly Sellers

6. Validation Of Molecular Diagnostic Assays And Quality Assurance and Control in the Veterinary Laboratory

Nick Saunders and Ian Sharp

 PART II - Molecular Detection and Identification of Animal Pathogens in Laboratorial Settings

7. Molecular Approaches To Recognize Relevant And Emerging Infectious Diseases In Animals

Fredrik Granberg, Oskar Karlsson, Mikael Leijon, Lihong Liu, and Sándor Belák

 8. Real Time Reverse Transcriptase PCR For The Detection Of Bluetongue Virus

Carrie Batten, Lorraine Frost, And Chris Oura

9. Nested And Multiplex Real Time PCR Using Dual-Labeled Probes: Detecting And Discriminating Mycobacterium Tuberculosis Complex Members In Cultures And Animal Tissues

Pedro Costa, Isabel Couto, Miguel Viveiros, and João Inácio

10. A Real-Time PCR Assay For The Diagnosis Of Gastrointestinal Nematode Infections of Small Ruminants

Florian Roeber, Aaron R. Jex, And Robin B. Gasser

 11. Improved Detection Of Mycobacterium Bovis In Bovine Tissues Using Immunomagnetic Separation Approaches

Irene R. Grant And  Linda D. Stewart

 12. Detection Of Fish Pathogens By Loop-Mediated Isothermal Amplification (LAMP) Technique

Hatem Soliman, Mona Saleh, And Mansour El-Matbouli

 13. Direct Detection Of Theileria Annulata In Bovine Blood Samples Using Standard And Isothermal DNA Amplification Approaches

Jacinto GomesAnd João Inácio

14. Reverse Line Blot Hybridization With Species-Specific Oligonucleotide Probes: Application To Piroplasms Detection

Ana Hurtado

 15. DNA Microarray-Based Detection Of Multiple Pathogens: Mycoplasma Spp. and Chlamydia Spp.

Christiane Schnee and  Konrad Sachse

 16. In Situ Hybridization With Labeled Probes: Assessment of African Swine Fever Virus In Formalin-Fixed Paraffin-Embedded Tissues

Maria Ballesterand Fernando Rodriguez

 17. Fluorescence In Situ Hybridization For The Tissue Detection of Bacterial Pathogens Associated With Porcine Infections

Henrik Elvang Jensen, Louise Kruse Jensen, Kristiane Barington, Susanne Pors, Thomas Bjarnsholt, and  Mette Boye

 18. Identification Of Animal Pasteurellaceae By MALDI-TOF Mass Spectrometry

Joachim Frey and Peter Kuhnert

 19. Gold Nanoparticles as a Potential Tool For Diagnosis of Fish Diseases

Mona Saleh, Hatem Soliman, and Mansour El-Matbouli

 20. Nucleic Acid Testing, New Platforms And Nanotechnology For Point-of-Decision Diagnosis Of Animal Pathogens

 Fernando Teles And Luís Pina Fonseca

 PART III - Molecular Profiling Of Veterinary Relevant Microbial Pathogens

21. Molecular Typing Tools: From Pattern Recognition To Genome-Based Algorithms

Konrad Sachse and  Petra Moebius

22. Analysis Of Campylobacter Jejuni And Campylobacter Coli Genotypes In Poultry Flocks By Restriction Fragment Length Polymorphism (RFLP) Analysis

Ana Cláudia Carreiraand Mónica V. Cunha

23. Pulsed-Field Gel Electrophoresis (PFGE): Application In Population Structure Studies Of Bovine Mastitis-Causing Streptococci

Ilda Santos-Sanches, Lélia Chambel, and Rogério Tenreiro

 24. Multiple-Locus Variable Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR And Multicolour Capillary Electrophoresis: Application To The Genotyping Of Brucella Species

Giuliano Garofolo

 25. Multilocus Sequence Typing (MLST): Markers For The Traceability Of Pathogenic Leptospira Strains

Ahmed Ahmed, Ana S. Ferreira, and Rudy A. Hartskeerl

 26. Single Nucleotide Polymorphisms Discrimination Using High-Resolution Melting Analysis For The Genotyping Of Bacillus Anthracis

Sylviane Derzelle

 27. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Analysis Of Members Of The Mycobacterium Tuberculosis Complex

Ana Botelho, Ana Canto, Célia Leão, and Mónica V. Cunha

 28. Rapid Microarray-Based Genotyping Of Chlamydia Spp. Strains From Clinical Tissue Samples

Konrad Sachse and Anke Ruettger

 29. Multiplexed Genotyping Of Bacillus Anthracis By Luminex Xmap Suspension Array

Simon Thierry and Sylviane Derzelle

 PART IV - Integrative Omics and High-Throughput Platforms to Unravel the Biology of Pathogens and Their Interaction With the Host

 30. Next-Generation Sequencing In Veterinary Medicine: How Can The Massive Amount Of Information Arising From High-Throughput Technologies Improve Diagnosis, Control and Management Of Infectious Diseases?

Steven Van Borm, Sándor Belák, Graham Freimanis, Alice Fusaro, Fredrik Granberg, Dirk Höper, Donald P. King, Isabella Monne, Richard Orton, and Toon Rosseel

31. Impact Of Next-Generation Technologies On Exploring Socio-Economically Important Parasites And Developing New Interventions

Cinzia Cantacessi, Andreas Hofmann, Bronwyn E. Campbell, and Robin B. Gasser

 32. Functional Genomics Of Tick Vectors Challenged With The Cattle Parasite Babesia Bigemina

Ana Domingos, Sandra Antunes, Margarita Villar, and José de la Fuente33. Metagenomic Approaches To Disclose Disease-Associated Pathogens: Detection Of Viral Pathogens In Honeybees

Fredrik Granberg, Oskar E. Karlsson, and Sándor Belák

34. Proteomics Characterization Of Tick-Host-Pathogen Interactions

Marina Popara, Margarita Villar, and José de la Fuente

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts