Description
Liposomes (2nd Ed., Softcover reprint of the original 2nd ed. 2017)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1522
Language: English
Subject for Liposomes:
Support: Print on demand
Publication date: 11-2016
Support: Print on demand
Description
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This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Liposomes: Methods and Protocols, Second Edition is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry.
Preface…
Table of Contents…
Contributing Authors…
1. Liposomes Came First: The Early History of Liposomology
Volkmar Weissig
2. Thin Film Hydration followed by Extrusion Method for Liposome Preparation
Hongwei Zhang
3. Preparation of DRV Liposomes
4. Method of Simultaneous Analysis of Liposome Components by HPTLC/FID
Sophia Hatziantoniou and Costas Demetzos
5. Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale
Brigitte Papahadjopoulos-Sternberg
6. Liposome Formulations of Hydrophobic Drugs
Reto A. Schwendener and Herbert Schott
7. A “Dock and Lock” Approach to Preparation of Targeted Liposomes
Marina V. Backer and Joseph M. Backer
8. Coupling of Ligands to the Liposome Surface by Click Chemistry
Maria Vittoria Spanedda, Marcella De Giorgi, Fatouma Saïd Hassane, Francis Schuber, Line Bourel-Bonnet, and Benoît Frisch
9. Elastic Liposomes for Topical and Transdermal Drug Delivery
11. Environmental Scanning Electron Microscope Imaging of Vesicle Systems
Yvonne Perrie, Habib Ali, Daniel J. Kirby, Afzal U. R. Mohammed, Sarah E. McNeil, and Anil Vangala
12. Liposome Biodistribution via Europium Complexes
Nathalie Mignet and Daniel Scherman
13. Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Preformed Liposomes for Diagnostic Imaging and Radionuclide Therapy<
Beth Goins, Ande Bao, and William T. Phillips
14. Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes
Suna Erdogan and Vladimir P. Torchilin
15. Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes
Nicolas Bertrand, Pierre Simard, and Jean-Christophe Leroux
16. Long-Circulating, pH-Sensitive Liposomes
Denitsa Momekova, Stanislav Rangelov, and Nikolay Lambov
17. Anionic pH-Sensitive Lipoplexes
Nathalie Mignet and Daniel Scherman
18. Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes
Edwin Pozharski
19. Preparation and Physical Characterization of DNA Binding Cationic Liposomes
Vaibhav Saxena
20. Fluorescence Resonance Energy Transfer (FRET) Based Analysis of Lipoplexes
Edwin Pozharski
21. Targeted Magnetic Liposomes Loaded with Doxorubicin
Pallab Pradhan, Rinti Banerjee, Dhirendra Bahadur, Christian Koch, Olga Mykhaylyk, and Christian Plank
22. Stable Discoidal Bicelles - A Platform of Lipid Nanocarriers for Cellular Delivery
Ying Liu, Yan Xia, Armin Tahmasbi Rad, Wafa Aresh and Mu-Ping Nieh
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
