Date Palm Biotechnology Protocols Volume I, Softcover reprint of the original 1st ed. 2017
Tissue Culture Applications
Methods in Molecular Biology Series, Vol. 1637

This two-volume book is a valuable resource to students, researchers, scientists, commercial producers, consultants and policymakers interested in agriculture or plant sciences particularly in date palm biotechnology. Date Palm Biotechnology Protocols, Volume 1: Tissue Culture and Applications is comprised of 27 chapters covering adventitious organogenesis, somatic embryogenesis, contamination, hyperhydricity, acclimatization, cell suspension, protoplast and bioreactors, genetic transformation secondary metabolites, and abiotic stress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Date Palm Biotechnology Protocols, Volume 1: Tissue Culture and Applications aims to supplement the previous volume and to provide precise stepwise protocols in the field of date palm biotechnology.

Part I   Adventitious Organogenesis

1                     

Cultivar-Dependent Direct Organogenesis of Date Palm from Shoot Tip Explants

Larbi Abahmane

2                     

Hussam S.M. Khierallah, Saleh M. Bader, and Makki A. Al-Khafaji 

3                     

Direct Organogenesis from Immature Female Inflorescence of Date Palm by Gradual Reduction of 2,4-D Concentration

Ezz El-Din G. Gadalla

4                     

Rehab Sidky

5                     

Direct Organogenesis and Indirect Somatic Embryogenesis by In Vitro Reversion of Mature Female Floral Buds to a Vegetative State

Eman M.M. Zayed

Part II   Somatic Embryogenesis

6                     

Enhanced Indirect Somatic Embryogenesis of Date Palm Using Low Levels of Seawater

Rania A. Taha

7                     

Enhanced Indirect Somatic Embryogenesis from Shoot-Tip Explants of Date Palm by Gradual Reductions of 2,4-D Concentration

Zeinab E. Zayed

8                     

Indirect Somatic Embryogenesis from Mature Inflorescence Explant of Date Palm

Ali M. Al-Ali, Chien-Ying Ko, Sultan A. Al-Sulaiman, Sami O. Al-Otaibi, Abd Almonem H. Al-Khamees, and Megahed H. Ammar

9                     

Indirect Somatic Embryogenesis of Date Palm Using Juvenile Leaf Explants and Low 2,4-D Concentration

Lotfi Fki, Walid Kriaa, Ameni Nasri, Emna Baklouti, Olfa Chkir, Raja B. Masmoudi, Alain Rival,  and  Noureddine Drira

10                 

Desiccation-Enhanced Maturation and Germination of Date Palm Somatic Embryos Derived from Cell Suspension Culture

Nazim Boufis, Khayreddine Titouh, and Lakhdar Khelifi

11                 

Desiccation and Cold Hardening of Date Palm Somatic Embryos Improve Germination

12                 

Histological Evidence of Indirect Somatic Embryogenesis from Immature Female Date Palm Inflorescences

Eman M. M. Zayed and Ola H. Abdelbar

Histological Analysis of Developmental Stages of Direct Somatic Embryogenesis Induced from In Vitro Leaf Explants of Date Palm

Part III   Contamination, Hyperhydricity and Acclimatization

14                 

Identifying and Controlling Contamination of Date Palm Tissue Cultures

Abeer H.E. Abd-El Kareim

15                 

Maiada M. El-Dawayati and Zeinab E. Zayed

16                 

Improvement of In Vitro Date Palm Plantlet Acclimatization Rate with Kinetin and Hoagland Solution

Mona M. Hassan

Part IV   Cell Suspension, Protoplast and Bioreactors

17                 

Plant Regeneration from Somatic Embryogenic Suspension Cultures of Date Palm

Mansour A.  Abohatem and Mohmmed Baaziz

18                 

Synchronization of Somatic Embryogenesis in Date Palm Suspension Culture Using Abscisic Acid

Hussain A. Alwael, Poornananda M. Naik, and Jameel M. Al-Khayri

19                 

Microcalli Induction in Protoplasts Isolated from Embryogenic Callus of Date Palm

Khayreddine Titouh, Nazim Boufis, and Lakhdar Khelifi

20                 

Temporary Immersion System for Date Palm Micropropagation

Ahmed Othmani, Chokri Bayoudh, Amel Sellemi and Noureddine Drira

21                 

Abdulminam H.A. Almusawi, Abdullah J. Sayegh, Ansam M.S. Alshanaw, and John L. Griffis, Jr.

Part V   Genetic Transformation

22                 

Genetic Transformation of Date Palm via Microprojectile Bombardment

23                 

Microprojectile Bombardment Transformation of Date Palm Using the Insecticidal Cholesterol Oxidase (ChoA) Gene

Mai A. Allam and Mahmoud M. Saker

24                 

Transient GUS Gene Expression in Date Palm Fruit Using Agroinjection Transformation Technique

Mohei El-Din M. Solliman, Hebaallah A. Mohasseb, Abdullatif A.  Al-Khateeb, Jameel M. Al-Khayri, and Suliman A. Al-Khateeb

Part VI    Secondary Metabolites and Abiotic Stress

25                 

Bioreactor Steroid Production and Analysis of Date Palm Embryogenic Callus

Sherif El-Sharabasy and Maiada El-Dawayati

26                 

Poornananda M. Naik and Jameel M. Al-Khayri

27                 

In Vitro Assessment of Abiotic Stress in Date Palm: Salinity and Drought

Jameel M. Al-Khayri, Poornananda M. Naik, and Hussain A. Alwael

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts