Doubled Haploid Technology, 1st ed. 2021
Volume 2: Hot Topics, Apiaceae, Brassicaceae, Solanaceae
Methods in Molecular Biology Series, Vol. 2288

This title offers 62 chapters divided among three volumes covering the latest topics dealing with Doubled Haploid (DH) technology, as well as methods to produce DHs in different species through different in vivo and in vitro approaches. Volume 2 discusses the latest hot topics in DH technology; protocols to produce DHs in four apiaceae, caraway, fennel, dill, and carrot; DH production in different brassicaceae including Brassica napus, Brassica rapa, and Brassica carinata; and techniques to produce DHs by different androgenesis-based methods in several members of the Solanaceae family such as eggplant, pepper, tobacco, and potato. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Doubled Haploid Technology, Volume 2: Hot Topics, Apiaceae, Brassicaceae, Solanaceae is a valuable resource for scientists and researchers looking to learn more about this interesting and developing field.

Preface…
Table of Contents…
Contributing Authors…

Part I Hot Topics in DH Technology

1. Albino Plant Formation in Androgenic Cultures: An Old Problem and New Facts
Iwona Żur, Monika Gajecka, Ewa Dubas, Monika Krzewska, and Iwona Szarejko

2. The Maize in planta Haploid Induction Lines: A Corner Stone for Doubled Haploid Technology
Nathanaël M.A. Jacquier, Laurine M. Gilles, Jean-Pierre Martinant, Peter M. Rogowsky and Thomas Widiez

3. The Use of Molecular Markers for Doubled Haploid Technology: From Academia to Plant Breeding Companies
Stine Due Tuvesson, Clas-Tomas Larsson, and Frank Ordon

4. Zygotic Embryogenesis in Flowering Plants
Houming Chen, Yingjing Miao, Kai Wang, and Martin Bayer

Part II Doubled Haploids in Apiaceae

5. Caraway (Carum carvi L.) - Anther Culture and Production of DH Plants
Iva Smýkalová and Jiří Horáček

6. Doubled Haploidy for Fennel (Foeniculum vulgare Mill.) and Dill (Anethum graveolens L.)
Alison M.R. Ferrie

7. Production of Homozygous Carrot (Daucus carota L.) Plants by Anther Culture
Waldemar Kiszczak, Maria Burian, Urszula Kowalska, and Krystyna Górecka

Part III Doubled Haploids in Brassicaceae

8. Doubled Haploid Production in High- and Low-Response Genotypes of Rapeseed (Brassica napus) through Isolated Microspore Culture
Patricia Corral-Martínez, Carolina Camacho-Fernández, Ricardo Mir, and Jose M. Seguí-Simarro

9. Brassica rapa L. ssp chinensis Isolated Microspore Culture Protocol
Daria Shumilina, Elena Kozar, Olga Chichvarina, Ksenia Korottseva, and Elena Domblides

10. Protocol for the Production of Doubled Haploid Plants of Brassica carinata
Annette Zatylny, Valerie Catinot, Tiina Bundrock, Siu Wah Wu, and Christina Eynck

11. Haploid and Doubled Haploid Plant Production in Brassica rapa L. subsp. pekinensis via Microspore Culture
Adela Adamus, Marek Szklarczyk, and Agnieszka Kiełkowska

12. Doubled Haploid Broccoli (Brassica olearacea var. italica) Plants from Anther Culture
Ali Ramazan Alan, Fevziye Celebi-Toprak, Alireza Lachin, Doguscan Yildiz, Volkan Gozen, and Gulay Besirli

13. Protocol of European Radish (Raphanus sativus L.) Microspore Culture for Doubled Haploid Plant Production
Elena Kozar and Elena Domblides

Part IV Doubled Haploids in Solanaceae

14. Anther and Isolated Microspore Culture in Eggplant (Solanum melongena L.)
Antonio Calabuig-Serna, Rosa Porcel, Patricia Corral-Martínez, and Jose M. Seguí-Simarro

15. Shed-Microspore Culture in Ornamental Peppers for Doubled Haploid Plant Production
Erin Ari

16. An Efficient Anther Culture on Double-Layered Media to Produce Doubled Haploid Plants of Pepper (Capsicum annum)
Ence Darmo Jaya Supena

17. Anther Culture in Sweet Pepper (Capsicum annuum L.)
Mireia Albiñana Palacios and Jose M. Seguí-Simarro

18. Optimized Protocol for Development of Androgenic Haploids and Doubled Haploids in FCV Tobacco (Nicotiana tabacum)
Salej Sood, Panuganti Satya Prasanna, Thamalampudi Venkata Reddy, and Saiprasad VS Gandra

19. Anther Culture of Potato: Method and Applications
Veli-Matti Rokka

20. Anther Culture of the Gametophytic Self-Incompatible Species Physalis ixocarpa Brot.
Rocío Escobar-Guzmán and Neftalí Ochoa-Alejo

21. Induction of Haploid Embryos in Datura metel by Anther Culture
Kolitha B. Wijesekara and Mohammed C.M. Iqbal

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains keynotes and implementation advice from the experts