Plant Cell Culture Protocols (4^th Ed., Softcover reprint of the original 4th ed. 2018)
Methods in Molecular Biology Series, Vol. 1815

Part I: Introduction

1. An Introduction to Plant Tissue Culture: Advances and Perspectives

Victor M. Loyola-Vargas and Neftalí Ochoa-Alejo

 

Part II: Cell Culture the Fundaments 2. Micropropagation in the XXI Century Jean Carlos Cardoso, Lee Tseng Sheng Gerald, and Jaime A. Teixeira da Silva  

3. Cellular and Morpho-histological Foundations of in vitro Plant Regeneration Diego Ismael Rocha, Lorena Melo Vieira, Andréa Dias Koehler, Wagner Campos Otoni   4. Bacterial Endophytes in Plant Tissue Culture: Mode of Action, Detection, and Control Mona Quambusch and Traud Winkelmann   5. Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture

Victor Gaba, Yehudit Tam, Danny Shavit, Benjamin S

 

6. Selection of Molecular Markers for the Estimation of Somaclonal Variation Octavio Martínez

 

7. Plant Tissue Culture. A Battle Horse in the Genome Editing Using CRISPR/Cas9 Víctor M. Loyola-Vargas and Randy N. Avilez-Montalvo

 

  Part III: Protocols 8. Micropropagation of Agave Species Benjamín Rodríguez-Garay and José Manuel Rodríguez-Domínguez

 

9. Protocol for the Micropropagation of Coconut from Plumule Explants Luis Sáenz, José Luis Chan, Maria Narvaez, and Carlos Oropeza

 

10. Micropropagation of Yucca Species Yessica López-Ramírez, Alejandra Palomeque-Carlín, Lucía Isabel Chávez Ortiz, Ma. de Lourdes de la Rosa-Carrillo, and Eugenio Pérez-Molphe-Balch  

11. Auxin Immunolocalization in Coffea canephora Tissues

Ruth E. Márquez-López, Ángela Ku-González, Hugo A. Méndez-Hernández, Rosa M. Galaz-Ávalos, and Víctor M. Loyola-Vargas

 

12. Somatic Embryogenesis in Common Bean Phaseolus vulgaris L. José Luis Cabrera-Ponce, Itzel Anayetzi González-Gómez, Claudia G. León-Ramírez, José A. Sánchez-Arreguín, and Alba E. Jofre y Garfias  

13. Induction of Somatic embryogenesis in Jatropha curcas Rosa M. Galaz-Avalos, Heydi G. Martínez-Sánchez, and Víctor M. Loyola-Vargas

 

14. In Vitro Proliferation of Female Buds for Induction of Somatic Embryogenesis from False Horn Plantain (AAB, Curraré) Rosa Maria Escobedo-Gracia-Medrano, Carlos Iván Cruz-Cárdenas, Lucila Aurelia Sánchez-Cach, José Roberto Ku-Cauich, and Wilma Aracely González-Kantún   15. Somatic Embryogenesis in Theobroma cacao L. Claudia Garcia, Jean-Philippe Marelli, Juan Carlos Motamayor, and Cristiano Villela   16. Somatic Embryogenesis of Quercus suber L. from Immature Zygotic Embryos Pilar S. Testillano, Aránzazu Gómez-Garay, Beatriz Pintos, and María C. Risueño   17. Cryotherapy: a Novel Method for Virus Eradication in Economically Important Plant Species Min-Rui Wang, Long Chen, Zhibo Zhang, Dag-Ragnar Blystad, and Qiao-Chun Wang   18. Cryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Technique Fernanda Vidigal Duarte Souza, Everton Hilo de Souza, Ergun Kaya, Lívia de Jesus Vieira, and Ronilze Leite da Silva   19. Cryopreservation of Pollen Grains of Pineapple and Other Bromeliads Fernanda Vidigal Duarte Souza, Everton Hilo de Souza, and Ronilze Leite da Silva   20. Application of in Casa Pollination and Embryo Rescue Techniques for Breeding of Agave Species Benjamín Rodríguez-Garay, Sigifredo López-Díaz, José Manuel Rodríguez-Domínguez, Antonia Gutiérrez-Mora, and Ernesto Tapia-Campos   21. Haploid and Doubled Haploid Plant Production in Carrot Using Induced Parthenogenesis and Ovule Excision in Vitro Agnieszka Kiełkowska, Adela Adamus, and Rafal Baranski   22. Using Flow Cytometry Analysis in Plant Tissue Culture Derived Plants Rosa María Escobedo-Gracia-Medrano, Martha Josefa Burgos-Tan, José Roberto Ku-Cauich, and Adriana Quiroz-Moreno   23. Procedure for Estimating the Tolerance and Accumulation of Heavy Metals Using Plant Cell Cultures Antonio Bernabé-Antonio, Amalia Maldonado-Magaña, María Elena Estrada-Zúñiga, Leticia Buendía-González and Francisco Cruz Sosa   24. Proteomics as a Tool to Study Molecular Changes During Plant Morphogenesis in Vitro André Luis Wendt dos Santos, Ricardo de Souza Reis, Angelo Schualb Heringer, Eny Iochevet Segal Floh, Claudete Santa-Catarina, and Vanildo Silveira   25. Proteomic Analysis of Non-Model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis and MALDI Mass Spectrometry Petra Peharec Štefanić, Mario Cindrić, and Biljana Balen   26. Chromatin Immunoprecipitation (ChiP) Protocol for the Analysis of Gene Regulation by Histone Modifications in Agave angustifolia Haw Rosa Us-Camas and Clelia De-la-Peña

 

27. Transcription Factors: Their Role in the Regulation of Somatic Embryogenesis in Theobroma cacao L. and other Species Claudia Garcia, Dahyana Britto, and Jean-Philippe Marelli   28. MicroRNA Expression and Regulation During Maize Somatic Embryogenesis Brenda Anabel López-Ruiz, Vasti Thamara Juárez-González, Elva Carolina Chávez-Hernández, and Tzvetanka D. Dinkova

 

29. Elaboration of Transcriptome during the Induction of Somatic Embryogenesis

Elsa Góngora-Castillo, Geovanny I. Nic-Can, Rosa M. Galaz-Ávalos, and Víctor M. Loyola-Vargas 30. Induction of Specialized Metabolism in in vitro Cultures of Capsicum chinense Jacq. Felipe A. Vázquez-Flota and María de Lourdes Miranda-Ham   31. Analysis of Terpenoid Indole Alkaloids, Carotenoids, Phytosterols, and NMR-Based Metabolomics for Catharanthus roseus Cell Suspension Cultures Mohd Zuwairi Saiman, Natali Rianika Mustafa, and Robert Verpoorte   32. Transformed Root Culture: from Genetic Transformation to NMR-based Metabolomics Andrey S. Marchev, Zhenya P. Yordanova,  and Milen I. Georgiev   33. Genetic Transformation of Pentalinon andrieuxii Tissue Cultures Yeseña Burgos-May, Elidé Avilés-Berzunza, Luis Manuel Peña-Rodríguez, and Gregorio Godoy-Hernández

 

 

Appendix

34. Appendix A: The Components of the Culture Media

Randy Avilez-Montalvo and Víctor M. Loyola-Vargas