Plant Cell Culture Protocols (3^rd Ed., Softcover reprint of the original 3rd ed. 2012)
Methods in Molecular Biology Series, Vol. 877

Cell culture methodologies have become standard procedures in most plant laboratories. Currently,  facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. ?Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.?Written in the successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

1. An Introduction to Plant Cell Culture

                Victor M. Loyola-Vargas and Neftalí Ochoa-Alejo

2. History of Plant Tissue Culture

                Trevor Thorpe

3. Callus, Suspension Culture and Hairy Roots. Induction, Maintenance, and Characterization

                Rosa M. Galáz-Ávalos, Sagrario Aguilar-Díaz, Pedro A. Xool-González, Silvia M. Huchín-May and Víctor M. Loyola-Vargas

4. Growth Measurements. Estimation of Cell Division and Cell Expansion

                Gregorio Godoy-Hernández and Felipe A. Vázquez Flota

5. Measurement of Cell Viability

                Lizbeth A. Castro-Concha, Rosa María Escobedo, and María de Lourdes Miranda-Ham

6. Pathogen and Biological Contamination Management in Plant Tissue Culture: Phyto-Pathogens, Vitro-Pathogens, and Vitro-Pests

                Alan C. Cassells

7. Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification and Encapsulation-Dehydration

                Zhenfang Yin, Long Chen, Bing Zhao, Yongxing Zhu, and Qiaochun Wang

8. The Study of in vitro Development in Plants: General Approaches and Photography

                Edward C. Yeung

9. Use of Statistics in Plant Biotechnology

                Michael E. Compton

10. Tissue Culture Methods for the Clonal Propagation and Genetic Improvement of Spanish Red Cedar (Cedrela Odorata)

                Yuri Peña-Rodríguez,  Juan  Juárez Gómez, José Antonio  González, and  Manuel L. Robert

11. Micropropagation of Banana

                Yıldız Aka Kaçar and Ben Faber

12. Liquid in vitro Culture for the Propagation of Arundo donax

                Miguel Ángel Herrera-Alamillo and Manuel L. Robert

13. Production of Haploids and Doubled Haploids in Maize

                Vanessa Prigge and Albrecht E. Melchinger

14. Maize Somatic Embryogenesis: Recent Features to Improve Plant Regeneration

                Verónica Garrocho-Villegas, Maria Teresa de Jesús-Olivera, and Estela Sánchez Quintanar

15. Improved Shoot Regeneration from Root Explants Using an Abscisic Acid Containing Medium

                Subramanian Paulraj and Edward C. Yeung

16. Cryopreservation of Shoot Tips and Meristems: An Overview of Contemporary Methodologies

                Erica E. Benson and Keith Harding

17. Anther Culture of Chili Pepper (Capsicum spp.)

                Neftalí Ochoa-Alejo

18. Production of Inter-Specific Hybrids in Ornamental Plants

                Juntaro Kato and Masahiro Mii

19. Plant Tissue Culture of Fast-Growing Trees for Phytoremediation Research

                José Luis Couselo, Elena Corredoira, Ana M. Vieitez, and Antonio Ballester       

20. Removing Heavy Metals by in vitro Cultures

                María del Socorro Santos-Díaz and María del Carmen Barrón-Cruz

21. Establishment of a Sanguinarine Producing Cell Suspension Culture of Argemone Mexicana  L. (Papaveraceae). Induction of  Alkaloid Accumulation

                Felipe A. Vázquez-Flota, Miriam Monforte-González, Cecilia Guízar-González, Jorge Rubio-Piña, and Karen Trujillo-Villanueva

22. Epigenetics, the Role of DNA Methylation on Tree Development

                Marcos Viejo Somoano, María E. Santamaría, José L. Rodríguez, Luis Valledor, Mónica Meijón, Marta Pérez, Jesús Pascual, Rodrigo Hasbún, Mario Fernández Fraga, María Berdasco, Peter E. Toorop, María J. Cañal, and Roberto Rodríguez Fernández

23. The Potential Roles of microRNAs in Molecular Breeding

                Qing Liu and Yue-Qin Chen

24. Determination of Histone Methylation in Mono- and Dicotyledons Plants

                Geovanny I. Nic-Can and Clelia De la Peña

25. Basic Procedures for Epigenetic Analysis in Plant Cell and Tissue Culture

                José L. Rodríguez, Jesús Pascual, Marcos Viejo Somoano, Luis Valledor, Mónica Meijón, Rodrigo Hasbún, Norma Yagüe Yrei, María E. Santamaría, Marta Pérez, Mario Fernádez Fraga, María Berdasco, Roberto Rodríguez Fernández, and María J. Cañal

26. Plant Tissue Culture and Molecular Markers

                María Tamayo-Ordoñez, Javier Huijara-Vasconselos, Adriana Quiroz-Moreno, Matilde Ortíz-García, and  Felipe Sánchez-Teyer

27. Biolistic- and Agrobacterium-Mediated Transformation Protocols for Wheat

                Cecília Tamáz-Nyitrai, Huw D. Jones, and László Tamás

28. Improved Genetic Transformation of Cork Oak (Quercus suber L.)

                Rubén Alvarez-Fernández and Ricardo-Javier Ordás

29. Organelle Transformation

                Anjanabha Bhattacharya, Anish Kumar, Nirali Desai, and Seema Parikh

30. Appendix A - The Components of the Culture Media

                Víctor M. Loyola-Vargas

31. Appendix B - Plant Biotechnology and Tissue Culture Resources in the Internet

                Víctor M. Loyola-Vargas

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras