Plant Embryogenesis, 1st ed. 2020
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2122

This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species. Chapters guide readers through genetic screens, phenotypic analysis, live imaging, transcriptional profiling, methods on other model and non-model species beyond Arabidopsis thaliana, and introduction to systems that allow to culture or produce zygotic and somatic embryos in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Embryogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

The chapter ?Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos? is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Part I: Mutagenesis and Genetic Screens

1. Genetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis and Maize

C. Stewart Gillmor, A. Mark Settles, and Wolfgang Lukowitz

2. EMS Mutagenesis of Arabidopsis Seeds

3. EMS Mutagenesis of Maize Pollen

A. Mark Settles

Part II: Microscopic Imaging and Phenotypic Analysis of Arabidopsis Embryos

4. Live-cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana

Minako Ueda, Yusuke Kimata, and Daisuke Kurihara

Che-Yang Liao and Dolf Weijers

6. From Stained Plant Tissues to Quantitative Cell Segmentation Analysis with MorphoGraphX

Merijn Kerstens, Soeren Strauss, Richard Smith, and Viola Willemsen

Part III: Expression Analysis and Transcriptomics

7. Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos

Katalin Páldi, Magda Mosiolek, and Michael D. Nodine

8. Manual Isolation of Living Early Embryos from Tobacco Seeds

Peng Zhao, Xuemei Zhou, Ce Shi, and Meng-xiang Sun

9. Profiling Transcriptomes of Manually Dissected Arabidopsis Embryos

10. Laser-assisted Microdissection of Plant Embryos for Transcriptional Profiling

Ana Marcela Florez-Rueda, Lucas Waser, and Ueli Grossniklaus

11. Cell-type-specific Transcriptomics in the Plant Embryo Using an Adapted INTACT Protocol

Joakim Palovaara and Dolf Weijers

Daniel Slane, Kenneth W. Berendzen, Janika Witthöft, and Gerd Jürgens

Part IV: Protein Interactions Studies

13. Visualizing Protein Associations in living Arabidopsis Embryo

Part V: Embryogenesis in Other Model and Non-Model Species

14. Imaging of Embryo Sac and Early Seed Development in Maize after Feulgen Staining

Kamila Kalinowska, Junyi Chen, and Thomas Dresselhaus

Min Chen, Anhthu Q. Bui, and Robert B. Goldberg

16. Microscopical Detection of Cell Death Processes during Scots Pine Zygotic Embryogenesis

Jaana Vuosku and Suvi Sutela

Part VI: In Vitro Systems to Study Embryogenesis

17. Regulation of Somatic Embryo Development in Norway Spruce

Sara von Arnold, Tianqing Zhu, Emma Larsson, Daniel Uddenberg, and David Clapham

18. In vitro Production of Zygotes by Electro-Fusion of Rice Gametes

Md Hassanur Rahman, Erika Toda, and Takashi Okamoto

19. Isolated Microspore Culture in Brassica napus

Patricia Corral-Martínez, Carolina Camacho-Fernández, and Jose M. Seguí-Simarro

20. Anther Culture in Eggplant (Solanum melongena L.)

Antonio Calabuig-Serna, Rosa Porcel, Patricia Corral-Martínez, and Jose M. Seguí-Simarro

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts