Plant Embryo Culture, 2011
Methods and Protocols
Methods in Molecular Biology Series, Vol. 710

A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book?s main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology? series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.

Zygotic Embryo Culture: An Overview.- In vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture.- Canola Zygotic Embryo Culture.- Immature Seeds and Embryos of Medicago truncatula Cultured In vitro.- In vitro Culture and Germination of Terrestrial Asian Orchid Seeds.- In vitro Culture of Coconut (Cocos nucifera L.) Zygotic Embryos.- Immature Embryo Rescue and Culture.- Chickpea Hybridization Using In vitro Techniques.- Muskmelon Embryo Rescue Techniques using In vitro Embryo Culture.- Phaseolus Immature Embryo Rescue Technology.- Wide Crossing in Lentil through Embryo Rescue.- Generation of Interspecific Hybrids of Trifolium Using Embryo Rescue Techniques.- Cryopreservation of Embryos: An Overview.- Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm.- Cryopreservation of Zygotic Embryo Axes and Somatic Embryos of European Chestnut.- Cryopreservation of Ilex Immature Zygotic Embryos.- The Use of Zygotic Embryos as Explants for In Vitro Propagation: An Overview.- Somatic Embryogenesis and Plant Regeneration in the Culture of Arabidopsis thaliana (L.) Heynh. Immature Zygotic Embryos.- Pine Somatic Embryogenesis Using Zygotic Embryos as Explants.- Micropropagation of Phalaenopsis Orchids via Protocorms and Protocorm-Like Bodies.- Genetic Transformation Protocols Using Zygotic Embryos as Explants: An Overview.- Genetic Transformation Using Maize Immature Zygotic Embryos.- Biolistic-Mediated Transformation Protocols for Maize and Pearl Millet Using Pre-Cultured Immature Zygotic Embryos and Embryogenic Tissue.- Agrobacterium tumefaciens-Mediated Genetic Transformation of Cereals Using Immature Embryos.

Presents detailed zygotic embryo isolation techniques with a wide variety of applications Serves as a quick, convenient reference source Includes tips from the labs of experts, well-versed in the methods and protocols provided