Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A
Methods in Enzymology Series

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A, Volume 621, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this volume covering Site-directed ethylation of membrane proteins for measuring conformational transitions in lipid bilayers, the Design and synthesis of fluorescent activity probes for protein phosphatases, Stains, Utilizing split-nanoLuc fragments as luminescent probes for protein solubility in living cells, SH2-domain based sensor for intracellular recognition of sulfo-tyrosine, DNA-encoded immunoglobulins for detection of parasites, An engineered TEV protease ? calmodulin fusion based sensor for neuronal calcium recording, and much more.

1. Targeted m⁶A reader proteins to study the epitranscriptome Simone Rauch and Bryan C. Dickinson 2. Synthetic gene expression circuits regulating sexual reproduction Nobuo Fukuda and Shinya Honda 3. Nucleotide resolution sequencing of N4-acetylcytidine in RNA Justin M. Thomas, Keri M. Bryson and Jordan L. Meier 4. A guide for drug inducible genome editing with HIT systems Chen Zhao, Shixian Wei and Yu Wang 5. A guide for drug inducible transcriptional activation with HIT systems Chen Zhao, Shixian Wei and Yu Wang 6. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination Jia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore 7. Salt-sensitive intein for large-scale polypeptide production Yi-Zong Lee and Shih-Che Sue 8. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls M. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg 9. Design, cloning and characterization of transcription factor-based inducible gene expression systems Erik K.R. Hanko, Nigel P. Minton and Naglis Malys 10. Design, construction, and validation of optogenetic proteins Colin P. O'Banion, Anwesha Goswami and David S. Lawrence 11. Overcoming component limitations in synthetic biology through transposon-mediated protein engineering Joshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg 12. Chemical biology of glycoproteins: From chemical synthesis to biological impact Yaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan 13. In bulla functional channel expression systems that mimic bacterial synthetic membranes Masayuki Iwamoto and Shigetoshi Oiki 14. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systems John E. Gadbery and Nicole S. Sampson 15. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases David Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin 16. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy Daniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia 17. Improved sensitivity and resolution of in-cell NMR spectra David S. Burz, Leonard Breindel and Alexander Shekhtman 18. High-throughput methods in aptamer discovery and analysis Kyle H. Cole and Andrej Lupták 19. Drop-in-well chamber for droplet interface bilayer with built-in electrodes Kazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki

Biochemists, chemists, microbiologists, microbiome, marine scientists, protein chemists, biotechnology, chemical biologists, blue biotech, natural product chemists, drug discovery

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology series
• Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics