Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

Language: English
Cover of the book Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

Subjects for Chemical Tools for Imaging, Manipulating, and Tracking...

Keywords

4-cyanotryptophan; 6p electrocyclization; Acid-brightening fluorescent protein (abFP); Acidic vesicles and organelles; Activatible fluorescent probes; Adenosine deaminase; Altered oscillations; Alzheimer"s disease; Amplitude; Amyloids; Amyloid-ß; Biocompatible reaction; Cell signaling; Chemoselective; Chromatin; Circadian rhythms; Cyanine dye; Diazaxanthilidene; Discovery assay; Discrimination; Environmentally-sensitive fluorophores; Epichaperome chemical probe; Expressed protein ligation; Fluorescence; Fluorescence imaging; Fluorescence microscopy; Fluorescent probe; Fluorescent probes; Fluorescent proteins; Fluorescent ribonucleoside; Fluoresecence polarization; Fluorogen; FRET; Genetic code expansion; Glycol auxiliary; hCRBPII; Hematologic malignancies; Histone; Histone demethylases; Hydrogen peroxide; Imaging; In cellulo; In vitro; Indirubin; Inhibitor; Isoindole; Isopeptide; Kaede; Live-cell; Live-cell imaging; Luminescent reporters; l-ß-(quinoline-6-yl) alanine; Qui; Macrocycle; Microscopy; Microscopy FRET; Mitotracker; Mouse models of cancer; Multiparameter flow cytometry; Native chemical ligation; Neurodegenerative diseases; N-hydroxysuccinimde ester; NIR fluorescence; No-wash Imaging; Oncogenic properties; Optical contrast agents; Ortho-phthalaldehyde; Peptide; Peptide cyclization; Peptide stapling; Period; PET; PHD; Photoaffinity; Photoconvertible; Photocrosslinking; Photoswitchable; Protein aggregation; Protein bioconjugation; Protein connectivity dysfunction; Protein fusion tags; Protein labeling; Ratiometric probe; Reactive oxygen species; Reader domains; Semi-synthesis; Single-cell proteome dysfunction detection; Small molecules; Spatiotemporal control; Spectroscopy; Synthesis; Three color FRET; Turn-ON fluorescence; Ubiquitin; Unnatural amino acid

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Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more.

    1. Fluorogenic detection of protein aggregates in live cells using the AggTag method

      2. Kwan Ho Jung and Xin Zhang

    2. Synthesis and application of a ratiometric probe for hydrogen peroxide

      3. Ruwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. Stains

    3. Protein labeling for FRET with methoxycoumarin and acridonylalanine

      4. Chloe M. Jones, Yarra Venkatesh and E. James Petersson

    4. Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assays

      5. Paul T. Ludford III and Yitzhak Tor

    5. Method to discriminate amyloids using fluorescent probes

      6. Kristine L. Teppang, Rachel S. Ehrlich and Jerry Yang

    6. Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives

      7. Hui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. Farkas

    7. Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancer

      8. John C. Widen, Martina Tholen, Joshua J. Yim and Matthew Bogyo

    8. Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles

      9. Nanxi Wang and Lei Wang

    9. 4-Cyanoindole-based fluorophores for biological spectroscopy and microscopy

      10. Arusha Acharyya, Ismail A. Ahmed and Feng Gai

    10. Dissecting contributions of catalytic and reader domains in regulation of histone demethylation

      11. Nektaria Petronikolou, James E. Longbotham and Danica Galonic Fujimori

    11. Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization

      12. Qing Zhang, Yue Zhang and Xuechen Li

    12. Chemical synthesis of di-ubiquitin modified histones for further biochemical studies

      13. Jia-Bin Li, Jun Liang and Changlin Tian

    13. Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies

      14. Swathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela Chiosis

    14. FlICk (fluorescent isoindole crosslinking) for peptide stapling

      15. Mihajlo Todorovic and David M. Perrin

    15. Selective protein N-terminal labeling with N-hydroxysuccinimide esters

      16. Hanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. Dempsey

    16. Covalent live-cell labeling of proteins using a photoreactive fluorogen

      17. Tewoderos M. Ayele, Steve D. Knutson and Jennifer M. Heemstra

    17. Photoconvertible diazaxanthilidene dyes for live cell imaging

      18. Rachel M. Lackner, Christopher L. Johnny and David M. Chenoweth

    18. No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag

Wei Sheng, James H. Geiger and Babak Borhan

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.
  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways