Immobilization of Enzymes and Cells (3^rd Ed., Softcover reprint of the original 3rd ed. 2013)
Methods in Molecular Biology Series, Vol. 1051

For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols,  in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.

1. New Opportunities for Immobilization of Enzymes

Jose M. Guisan

3. Glutaraldehyde Mediated Protein Immobilization

Fernando López-Gallego, Jose M. Guisán,and  Lorena Betancor

4. Immobilization of Enzymes on Mono-and Hetero-Functional Epoxy-Activated Supports

Cesar Mateo, Valeria Grazu, and Jose M. Guisan

5. Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Groups

Fernando López-Gallego,  Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Cesar Mateo and  Jose M. Guisan

6. Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports

Cesar Mateo, Gloria Fernandez-Lorente, Rocha-Martin, J., Bolivar, Juan, M., Jose M. Guisan

7. Reversible Covalent Immobilization of Enzymes Via Disulfide Bonds

Karen Ovsejevi , Carmen Manta, and  Francisco Batista-Viera

8. Immobilization of Candida rugosa Lipase on Superparamagnetic Fe₃O₄ Nanoparticles for Biocatalysis in Low Water Media

Joyeeta Mukherjee, Kusum Solanki, and Munishwar Nath Gupta

9. Immobilization of Enzymes by Bioaffinity Layering

Veena Singh, Meryam Sardar, and  Munishwar Nath Gupta

10. Immobilization of Enzymes on Magnetic Beads through Affinity Interactions

Audrey Sassolas, Akhtar Hayat and Jean-Louis Marty

11. Tips for the Functionalization of  Nanoparticles with Antibodies

Ester Polo, Sara Puertas, María Moros, Pilar Batalla, José M. Guisán, Jesús M. de la Fuente, Valeria Grazú

12. Design and Characterization of Functional Nanoparticles for Enhanced Bio-Performance

Pablo del Pino, Scott G. Mitchell & Beatriz Pelaz

13. Immobilization of Enzymes on Ethynyl-modified Electrodes Via Click Chemistry

Akhtar Hayat, Audrey Sassolas, Amina Rhouati, Jean-Louis Marty

14. Modification of Carbon Nanotube Electrodes with 1-pyrenebutanoic Acid, Succinimidyl Ester for Enhanced Bioelectrocatalysis

Guinevere Strack, Robert Nichols, Plamen Atanassov, Heather R. Luckarift, and Glenn R. Johnson

15. Enzyme Immobilization by Entrapment within a Gel Network

Audrey Sassolas, Akhtar Hayat and Jean-Louis Marty

16. Practical Protocols for Lipase Immobilization via Sol-Gel Techniques

Manfred T. Reetz

17. Improving Lipase Activity by Immobilization and Post-Immobilization Strategies

Jose M. Palomo, Marco Filice, Oscar Romero and Jose M. Guisan

18. High Activity Preparations of Lipases and Proteases for Catalysis in Low Water Containing Organic Solvents and Ionic Liquids

Ipsita Roy, Joyeeta Mukherjee_,  and Munishwar Nath Gupta

19. Biomedical Applications of Immobilized Enzymes: An Update

Marta Pastor, Amaia Esquisabel, and José Luis Pedraz

Susan R. Sizemore, Robert Nichols, Randi Tatum, Plamen Atanassov, Glenn R. Johnson, and Heather R. Luckarift

21. Encapsulation of Cells in Alginate Gels

Pello Sánchez, Rosa María Hernández, José Luis Pedraz, and Gorka Orive

22. Microalgal Immobilization Methods

Ignacio Moreno-Garrido

23. Therapeutic Applications of Encapsulated Cells

Argia Acarregui, Gorka Orive, José Luis Pedraz, and Rosa María Hernández

24. Whole Cell Entrapment Techniques

Jorge A. Trelles and Cintia W. Rivero

Demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras