Ion Mobility-Mass Spectrometry, 1st ed. 2020
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2084

This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Fundamentals of Ion Mobility-Mass Spectrometry for the Analysis of Biomolecules.- Utilizing Drift Tube Ion Mobility Spectrometry for the Evaluation of Metabolites and Xenobiotics.- Untargeted Differential Metabolomics Analysis Using Drift Tube Ion Mobility-Mass Spectrometry.- Drift Tube Ion Mobility-Mass Spectrometry for Non-Targeted 'Omics.- The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines.- Traveling Wave Ion Mobility-Mass Spectrometry to Enhance the Detection of Low Abundance Features in Untargeted Lipidomics.- Lipidomics by HILIC-Ion Mobility-Mass Spectrometry.- A Workflow for the Identification of Mycotoxin Metabolites Using Liquid Chromatography–Ion Mobility-Mass Spectrometry.- Quantitation of Cyclosporin A in Cell Culture Media by Differential Mobility Mass Spectrometry (DMS-MS/MS).- An Analytical Perspective on Protein Analysis and Discovery Proteomics by Ion Mobility-Mass Spectrometry.- Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics.- Liquid Extraction Surface Analysis (LESA) High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Mass Spectrometry for In Situ Analysis of Intact Proteins.- Ion Mobility-Mass Spectrometry of Glycoconjugates.- Desorption Atmospheric Pressure Photoionization Coupled with Ion Mobility-Mass Spectrometry.- Metabolomic Profiling of Adherent Mammalian Cells In Situ by LAESI-MS with Ion Mobility Separation.- Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled to Ion Mobility.- The Use of LipidIMMS Analyzer for Lipid Identification in Ion Mobility-Mass Spectrometry-Based Untargeted Lipidomics.- MolFind²: A Protocol for Acquiring and Integrating MS³ Data to Improve In-Silico Chemical Structure Elucidation for Metabolomics.- Collision Cross Section Calculations Using HPCCS.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts