Molecular Typing of Blood Cell Antigens, Softcover reprint of the original 1st ed. 2015
Methods in Molecular Biology Series, Vol. 1310

This volume aims to bring together a variety of protocols useful for DNA-based typing of blood cell antigens. Protocols range from simple approaches with low technical complexity to highly sophisticated modern developments. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Typing of Blood Cell Antigens summarizes contributions from leading scientist in the field DNA typing for blood cell antigens.

1. Validation Of Genotyping Protocols for Diagnostic Use

Paul Metcalfe

2. High Resolution Melting Analysis Of Single Nucleotide Polymorphisms

Carol M. Bruzzone And Clifford J. Steer

3. High-Speed Droplet-Allele-Specific Polymerase Chain Reaction for Genotyping Of Single Nucleotide Polymorphisms

Kazuyuki Matsuda And Takayuki Honda

4. Blood Grouping Based On Pcr Methods and Agarose Gel Eletrophoresis

Ana Maria Sell And Jeane Eliete Laguila Visentainer

5. Parallel Donor Genotyping For 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput Maldi-Tof Mass Spectrometry

Stefan Meyer, Nadine Trost, Beat M. Frey, And Christoph Gassner

6. Pcr With Sequence-Specific Primers for Typing of Diallelic Blood Groups

Gabriele Rink, Erwin A. Scharberg, and Peter Bugert

7. High-Resolution Melting Analysis for Genotyping Duffy Blood Group Antigens

Ewa Łukasik, Kazimiera Waśniowska, Magdalena Grodecka,Edyta Majorczyk, and Marcin Czerwiński

8. Molecular Rhd-Rhce Analysis by Multiplex Pcr of Short Fluorescent Fragments

Yann Fichou and Claude Férec

9. Microarrays in Blood Group Genotyping

Stephanie A. Boccoz, Gaëlle Le Goff, Loïc J. Blum, and Christophe A. Marquette

10. Next Generation Sequencing for Antenatal Prediction of Kel1 Blood Group Status

Klaus Rieneck, Frederik Banch Clausen, and Morten Hanefeld Dziegiel

11. 454-Sequencing™ For The Kel, Jr And Lan Blood Groups

Carola Wieckhusen and Peter Bugert

12. Non-Invasive Prenatal Blood Group Genotyping

Andrea Doescher and Thomas H. Müller

13.  Genotyping Of Human Platelet Antigens By Beadchip Microarray Technology

Gerald Bertrand and Fabiana Conti

14. Sequence-Based Typing For Platelet Alloantigens

Shun-Chung Paiand Liang-In Lin

15. Miniaturized Technology For Dna Typing: Cassette Pcr

Dammika P. Manage and Linda M. Pilarski

16. Geno- And Phenotyping Of Human Neutrophil Antigens

Angelika Reil and Jürgen Bux

17. Allelic Discrimination by Taqman-Pcr For Genotyping of Human Neutrophil Antigens

Rudi Steffensen, John Baech, and Kaspar R. Nielsen

18. Novel Approaches And Technologies in Molecular Hla Typing

Paul P. J. Dunn

19. Luminex-Based Methods in High Resolution Hla Typing

Manuela Testi and Marco Andreani

Sebastian Boegel Jelle Scholtalbers, Martin Löwer, Ugur Sahin, and John C. Castle

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts