Brain Development (2nd Ed., 2nd ed. 2020)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2047

Language: English

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Brain Development
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Brain Development
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527 p. · 17.8x25.4 cm · Hardback
This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain.

Part I: Invertebrate Models

 

1. Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra

            Wanda Buzgariu, Marie-Laure Curchod, Chrystelle Perruchoud, and Brigitte Galliot

 

2. Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis

            Jamie A. Havrilak and Michael J. Layden

 

3. Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis

            Fabian Rentzsch, Eduard Renfer, and Ulrich Technau

 

4. Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System

            Elena Perea-Atienza, Brenda Gavilán, Simon G. Sprecher, and Pedro Martinez

 

5. Immunostaining of the Embryonic and Larval Drosophila Brain

            Frank Hirth and Danielle C. Diaper

 

6. Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila

            David Jussen and Rolf Urbach

 

7. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling

            Karoline F. Kraft and Rolf Urbach

 

8. Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update

            Emma L. Powell and Iris Salecker

 

9. Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain

            Karolina Miszczak and Boris Egger

 

10. CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila

            Cornelia Fritsch and Simon G. Sprecher

 

11. The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development

            Max S. Farnworth, Kolja N. Eckermann, Hassan M.M. Ahmed, Dominik S. Mühlen, Bicheng He, and Gregor Bucher

 

12. A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum

            Marita Büscher, Georg Oberhofer, Natalia Carolina Garcia-Perez, and Gregor Bucher

 

13. Immunohistochemistry and Fluorescent Whole Mount RNA In SituHybridization in Larval and Adult Brains of Tribolium

            Vera S. Hunnekuhl, Max S. Farnworth, Bicheng He, and Gregor Bucher

 

14. X-Ray Microscopy of the Larval Crustacean Brain

            Jakob Krieger and Franziska Spitzner

 

15. Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain

            Steffen Harzsch and Caroline Viertel

 

16. Immunostainings in Nervous System Development of the Nematode C. elegans

            Janet S. Duerr

 

17. Methods in Brain Development of Molluscs

            Andreas Wanninger and Tim Wollesen

 

18. A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization

            Clare Hudson

 

19. Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum

            Yann Le Petillon, Stéphanie Bertrand, and Héctor Escrivà

 

Part II: Vertebrate Models

 

20. In Situ Hybridization and Immunostaining of Xenopus Brain

            Kai-li Liu, Xiu-mei Wang, Zi-long Li, Ying Liu, and Rong-qiao He

 

21. Morpholino Studies in Xenopus Brain Development

            Jennifer E. Bestman and Hollis T. Cline

 

22. Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish

            Gilbert Lauter, Iris Söll, and Giselbert Hauptmann

 

23. Live Morphometric Classification of Sensory Neurons in Larval Zebrafish

            Gema Valera and Hernán López-Schier

 

24. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain

            Richard P. Tucker, Tatsuto Ishimaru, and Qizhi Gong

 

25. Gene Silencing in Chicken Brain Development

            Georgia Tsapara, Irwin Andermatt, and Esther T. Stoeckli

 

26. Transplantation of Neural Tissue: Quail-Chick Chimeras

            Andrea Streit and Claudio D. Stern

 

27. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development

            Jinling Liu and Aimin Liu

 

28. The Cre/Lox System to Assess the Development of the Mouse Brain

            Claudius F. Kratochwil and Filippo M. Rijli

 

29. In Utero Electroporation to Study Mouse Brain Development

            Emilie Pacary and François Guillemot

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts