Cancer Cell Signaling (2^nd Ed., Softcover reprint of the original 2nd ed. 2014)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1165

Cancer Cell Signaling: Methods and Protocols, Second Edition brings together the most recent advances in cancer cell signaling knowledge along with recent progress in the development of novel strategies and experimental cell models to study human cancer. Opening with a section on targeting cancer cell metabolism as an anticancer strategy, this detailed volume continues with parts describing protocols to study epigenetic control of cancer, protocols to study cancer metastasis, as well as two new techniques for cancer study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions for their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Second Edition is an ideal guide to the techniques that will aid researchers in exploiting our current knowledge in order to make a profound impact in cancer prevention and treatment.

Part I: Targeting Cancer Cell Metabolism as Anticancer Therapy

1. Use of Biguanides to Improve Response to Chemotherapy

            Vlad C. Sandulache, Liangpeng Yang, and Heath D. Skinner

2. Evaluating Response to Metformin/Cisplatin Combination in Cancer Cells Via Metabolic Measurement and Clonogenic Survival

            Sang Hyeok Woo, Vlad C. Sandulache, Liangpeng Yang, and Heath D. Skinner

3. Quantifying the Autophagy-Triggering Effects of Drugs in Cell Spheroids with Live Fluorescence Microscopy

            Nariman Ansari, Stefanie Müller, Katharina Hötte, Stefanie Rakel, Patrick Antonietti, Donat Kögel, Ernst H.K. Stelzer, and Francesco Pampaloni

Part II: Epigenetic Control of Cancer

4. Interdependency Between Genetic and Epigenetic Regulatory Defects in Cancer

            Félix Recillas-Targa

5. Experimental Strategies to Manipulate the Cellular Levels of the Multifunctional Factor CTCF

            Edgar González-Buendía, Rosario Pérez-Molina, Erandi Ayala-Ortega, Georgina Guerrero, and Félix Recillas-Targa

6. Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers

            Fermín Mar-Aguilar, Cristina Rodríguez-Padilla, and Diana Reséndez-Pérez

7. Prostate Cancer Detection Using a Non-Invasive Method for Quantifying miRNAs

            Mauricio Rodríguez-Dorantes,  A. Ivan  Salido-Guadarrama, and Pilar García-Tobilla

8. DNA Methylation Analysis of Steroid Hormone Receptor Genes

            Ignacio Camacho-Arroyo, Valeria Hansberg-Pastor, and Mauricio Rodríguez-Dorantes

9. Control of Oncogenic miRNA Function by Light-Activated miRNA Antagomirs

            Colleen M. Connelly and Alexander Deiters

10. Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling

            Yi Feng, Xiaowen Hu, Youyou Zhang, Dongmei Zhang, Chunsheng Li, and Lin Zhang

Part III: Metastasis Promotion

11. Microvesicles as Mediators of Intercellular Communication in Cancer

            Marc A. Antonyak and Richard A. Cerione

12. In Vivo Rat Model to Study Horizontal Tumor Progression

            Catalina Trejo-Becerril, Enrique Pérez-Cárdenas, and Alfonso Dueñas-González

13. An In Vivo Model to Study the Effects of Tumoral Soluble Factors on the Vascular Permeability in Mice

            Cesar Alejandro Guzmán-Pérez, Alfredo Ibarra-Sánchez, José Luis Ventura-Gallegos, Claudia González-Espinosa, Jonathan García-Román, and Alejandro Zentella-Dehesa

14. Use of the Tumor Repressor DEDD as a Prognostic Marker of Cancer Metastasis

            Qi Lv, Fang Hua, and Zhuo-Wei Hu

15. Zebrafish-Based Systems Pharmacology of Cancer Metastasis

            Yasuhito Shimada, Yuhei Nishimura, and Toshio Tanaka

Part IV: New Technologies in the Study of Cancer

16. A Flow Cytometry-Based Assay for the Evaluation of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) in Cancer Cells

            Nohemí Salinas-Jazmin, Emiliano Hisaki-Itaya, and Marco A. Velasco-Velázquez

17. Gene Disruption Using Zinc Finger Nuclease Technology

            Sara Granja, Ibtissam Marchiq, Fatima Baltazar, and Jacques Pouysségur

Includes cutting-edge methods and protocols for studying cancer cell signaling

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts