Hydrogen Peroxide and Cell Signaling, Part C
Methods in Enzymology Series

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy.

1.  
   1. The Biological Chemistry of Hydrogen Peroxide
      
   2. Reactive Oxygen Species in the Activation of MAP Kinases
      
   3. Hydrogen Peroxide Signaling Mediator in the Activation of p38 MAPK in Vascular Endothelial Cells
      
   4. In vivo Imaging of Nitric Oxide and Hydrogen Peroxide in Cardiac Myocytes
      
   5. Methods for Studying Oxidative Regulation of Protein Kinase C
      
   6. P66Shc, Mitochondria and the Generation of Reactive Oxygen Species
      
   7. Detecting Disulfide Bound Complexes and the Oxidative Regulation of Cyclic Nucleotide Dependent Protein Kinases by H₂O₂
      
   8. Redox Regulation of Protein Tyrosine Phosphatases: Methods for Kinetic Analysis of Covalent Enzyme Inactivation
      
   9. Activation of Nrf2 by H₂O₂: De novo Synthesis versus Nuclear Translocation
      
   10. H₂O₂ in the Induction of NF-k B-Dependent Selective Gene Expression
       
   11. Detection of H2O2-Mediated Phosphorylation of Kinase-Inactive PDGFRa
       
   12. Genetic Modifier Screens to Identify Components of a Redox-Regulated Cell Adhesion and Migration Pathway
       
   13. Investigating the Role of Reactive Oxygen Species in Regulating Autophagy
       
   14. H2O2: A Chemoattractant?
       
   15. Measuring Mitochondrial Uncoupling Protein-2 Level and Activity in Insulinoma Cells
       
   16. Effects of H2O2 on Insulin Signaling and the Glucose Transport System in Mammalian Skeletal Muscle
       
   17. Monitoring of Hydrogen Peroxide and Other Reactive Oxygen and Nitrogen Species Generated by Skeletal Muscle
       

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers hydrogen peroxide and cell signaling
• Contains chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy