Meningococcal Vaccines, Softcover reprint of the original 1st ed. 2001
Methods and Protocols
Methods in Molecular Medicine Series, Vol. 66

Since the first recognition of outbreaks of cerebrospinal or spotted fever at the end of the nineteenth and the beginning of the twentieth centuries, the menace of the meningococcus has been high on the list of public health prio- ties. Few if any pathogens surpass the meningococcus in the rapidity and sev- ity with which it devastates previously healthy individuals. The challenge of understanding the biology of this fascinating microbe is immense, but few will doubt that successful control of meningococcal meningitis and septicemia will only transpire through the application of a body of extraordinary detailed inf- mation, including key minutiae of its molecular biology. In the first of two companion volumes, Meningococcal Vaccines, the team of experts gathered by Andy Pollard and Martin Maiden converge to p- vide an impressive accumulation of molecular tools with which to lay bare the secrets of the meningococcus. The results of this ambitious and welcome v- ume represent an exciting and much needed resource for all of us in the field.

Meningococcal Vaccines and Vaccine Developments Ian M. Feavers Immune Response and Host-Pathogen Interactions Andrew J. Pollard and David Goldblatt Purification of Capsular Polysaccharide Qingling Yang and Harold Jennings Protein-Polysaccharide Conjugation Zhongwu Guo and Harold Jennings N-Propionylation Zhongwu Guo and Harold Jennings Outer Membrane Protein Purification, armen Arigita, Wim Jiskoot, Matthijs R. Graaf, and Gideon F. A. Kersten Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease Carl E. Frasch, Loek van Alphen, Johan Holst, Jan T. Poolman, and Einar Rosenqvist Methods for Manipulation of Transferrin-Binding Proteins Leanne M. DeWinter and Anthony B. Schryvers Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. meningitidis Outer Membrane Proteins Richard F. Collins and Jeremy P. Derrick Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics Janet Suker and Bambos M. Charalambous Construction of porA Mutants Peter van der Ley and Loek van Alphen Construction of LPS Mutants Peter van der Ley and Liana Steeghs Recombinant Proteins in Vaccine Development Myron Christodoulides, Keith A. Jolley, and John E. Heckels Identification of Peptides that Mimic N. meningitidis LOS Epitopes Via the Use of Combinatorial Phage-Display Libraries Paul J. Brett, Ian M. Feavers, and Bambos M. Charalambous Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity Alexei A. Delvig, John H. Robinson, and Lee Wetzler Early Experiences with Nasal Vaccines against Meningococcal Disease Johan Holst and Bjørn Haneberg Animal Models for Meningococcal Disease Andrew R. Gorringe, Karen M.Reddin, Pierre Voet, and Jan T. Poolman Determination of Antibody Responses to Meningococcal Antigens by ELISA Einar Rosenqvist, Helena Käyhty, and Andrew J. Pollard Immunoblot Analysis of Sera from Patients and Vaccinees Elisabeth Wedege Serogroup B and C Serum Bactericidal Assays Ray Borrow and George M. Carlone A Modified ELISA for Measurement of High-Avidity IgG Antibodies to Meningococcal Serogroup C Polysaccharide that Correlate with Bactericidal Titers Dan M. Granoff and John J. Donnelly Whole-Blood Model Catherine A. Ison Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry Terje E. Michaelsen and Audun Aase T-Cell Responses Against Meningococcal Antigens Lisbeth Meyer Næss, Fredrik Oftung, Audun Aase, Terje E. Michaelsen and Andrew J. Pollard CD4 T-Cell Epitope Mapping Alexei A. Delvig and John H. Robinson Epitope Mapping John E. Heckels and Myron Christodoulides Meningococcal Vaccine Trials Paddy Farrington and Elizabeth Miller The Introduction of Group C Conjugate Meningococcal Vaccine into the UK David M. Salisbury