Metabolic Profiling, 2011
Methods and Protocols
Methods in Molecular Biology Series, Vol. 708

At the intersection of metabolite analysis, metabolic fingerprinting, and metabolomics, the study of metabolic profiling has evolved steadily over the course of time as have the methods and technologies involved in its study. In Metabolic Profiling: Methods and Protocols, expert researchers in the field present protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. Comprised of the most essential techniques, this volume covers topics from inborn errors of metabolism and drug metabolite analysis to nuclear magnetic resonance metabolic profiles. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metabolic Profiling: Methods and Protocols serves as a resource for both established and new investigators in this vital and ever-developing field.

Origins of Metabolic Profiling.- Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism.- Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry.- Analysis of Organic Acids and Acylglycines for the Diagnosis of Related Inborn Errors of Metabolism by GC- and HPLC-MS.- HPLC Analysis of Purines and Pyrimidines for the Clinical Biochemical Diagnosis of Related Inborn Errors of Metabolism.- Bile Acid Analysis in Various Biological Samples Using Ultra Performance Liquid Chromatography/Electrospray Ionization-Mass Spectrometry (LC/ESI-MS).- Analysis of Glycolytic Intermediates with Ion Chromatography- and Gas Chromatography-Mass Spectrometry.- Analysis of the Citric Acid Cycle Intermediates Using Gas Chromatography-Mass Spectrometry.- Quantification of Pentose Phosphate Pathway (PPP) Metabolites by Liquid Chromatography-Mass Spectrometry (LC-MS).- High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)-Based Drug Metabolite Profiling.- Gas Chromatography-Mass Spectrometry (GC-MS)-Based Metabolomics.- The Use of Two Dimensional Gas Chromatography-Time-of-Flight-Mass Spectrometry (GCxGC-TOF-MS) for Metabolomic Analysis of Polar Metabolites.- LC-MS-Based Metabolomics.- Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)-Based Metabolomics.- Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues.- Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS)-Based Shotgun Lipidomics.- Processing and Analysis of GC/LC-MS-Based Metabolomics Data.- Nuclear Magnetic Resonance (NMR)-Based Drug Metabolite Profiling.- Nuclear Magnetic Resonance (NMR)-Based Metabolomics.- Slow Magic Angle Sample Spinning: A Non- or Minimal-Invasive Method for High Resolution 1H Nuclear Magnetic Resonance (NMR) Metabolic Profiling.- Processing and Modeling of Nuclear Magnetic Resonance (NMR) Metabolic Profiles.

Illustrates the evolution of metabolic profiling from single molecule profiling to global metabolome profiling Features cutting-edge protocols, presented from the perspective of leading experts in the field Includes valuable notes highlighting tips on troubleshooting and avoiding known methodological pitfalls Includes supplementary material: sn.pub/extras