Description
Virus Hybrids as Nanomaterials, Softcover reprint of the original 1st ed. 2014
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1108
Coordinators: Lin Baochuan, Ratna Banahalli
Language: EnglishSubject for Virus Hybrids as Nanomaterials:
Publication date: 08-2016
Support: Print on demand
235 p. · 17.8x25.4 cm · Hardback
Description
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In Virus Hybrids as Nanomaterials: Methods and Protocols expert researchers in the field detail many of the methods used to study virus for medial and nonmedical applications. These include methods and techniques for genetically engineering viruses for therapeutic purpose and vaccine production, chemically modified viruses for virus-templated nanoparticles production, and genetically engineered or chemically modified viral particles as imaging agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Virus Hybrids as Nanomaterials: Methods and Protocols seek to aid new researchers to get involved in this multidisciplinary area.
Part I: Virus Hybrids as Therapeutic/Vaccine Materials
1. Genetic Engineering and Chemical Conjugation of Potato Virus X
Karin L. Le, Kerstin Uhde-Holzem, Rainer Fischer, Ulrich Commandeur, Nicole F. Steinmetz
2. Genetically Engineering Adenoviral Vectors for Gene Therapy
Lynda Coughlan
3. Simulated Digestion for Testing the Stability of Edible Vaccine, Cucumber mosaic virus (CMV) Chimeric Particle Display Hepatitis C virus (HCV) Peptide
Vitti Antonella, Nuzzaci Maria, Condelli Valentina, Piazzolla Pasquale
4. Using Phage as a Platform to Select Cancer Cell Targeting Peptides
Xin Li, Chuanbin Mao
5. Bacteriophage T4 Capsid Packaging and Unpackaging of DNA and Proteins
Julienne M. Mullaney and Lindsay W. Black
Part II: Virus as building Block
6. Templated Mineralization by Charge-modified Cowpea Mosaic Virus
Alaa A. A. Aljabali and David J. Evans
7. Polyelectrolyte-modified Cowpea Mosaic Virus for the Synthesis of Gold Nanoparticles
Alaa A. A. Aljabali and David J. Evans
8. Tobacco Mosaic Virus Capsid Protein as Targets for the Self-assembly of Gold Nanoparticles
Omar K. Zahr, Amy Szuchmacher Blum
9. Tobacco Mosaic Virus Assembled High Aspect Ratio Surfaces
Adam D. Brownand James N. Culver
10. Phage as a Template to Grow Bone Mineral Nanocrystals
Binrui Cao, Hong Xu, and Chuanbin Mao
Part III: Virus Hybrids as Imaging Materials
11. Genetic Engineering and Characterisation of Cowpea Mosaic Virus Empty Virus-like Particles
Frank Sainsbury, Pooja Saxena, Alaa AA Aljabali, Keith Saunders, David J Evans, George P Lomonossoff
12. A Programmable Fluorescent Viral Nanoblock: Sensing Made Easy in a Single Step
Carissa M. Soto
13. Chemical Modification of the Inner and Outer Surfaces of Tobacco Mosaic Virus (TMV)
Michael A. Bruckman and Nicole F. Steinmetz
14. Engineered Bacteriophage T4 Nanoparticles for Cellular Imaging
Jinny L. Liu and Kelly L. Robertson
15. Phage-displayed Single Domain Antibodies as Recognition Elements
Ellen Goldman and Scott Walper
16. Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancer
Cho, C.-F., Sourabh, S., Simpson, E.J., Steinmetz, N.F., Luyt, L.G., and Lewis, J.D.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts