Description
Enzyme Engineering and Evolution: Specific Enzyme Applications
Methods in Enzymology Series
Language: EnglishSubjects for Enzyme Engineering and Evolution: Specific Enzyme...:
Keywords
Enzyme; Engineering; Evolution; Site-directed recombination; Diversity; proteins
312 p. · 15x22.8 cm · Hardback
Description
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1. Engineering lytic polysaccharide monooxygenases (LPMOs)
Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink
2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases
Daniela M. Carminati, Eric J. Moore and Rudi Fasan
3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries
Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer
4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution
Duncan S. Macdonald and Donald Hilvert
5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling
Lukas Deweid, Adem H. Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier
6. Engineering aldolases for asymmetric synthesis
Mikael Widersten
7. Ultra-high throughput screening for novel protease specificities
Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke
8. Directed evolution of transcriptional switches using dual-selector systems
Yuki Kimura and Daisuke Umeno
9. Methods for competitive enrichment and evaluation of superior DNA ligases
Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley
10. Directed evolution of custom polymerases using droplet microfluidics
Derek Vallejo, Ali Nikoomanzar and John C. Chaput
11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution
Pu Xue, Tong Si and Huimin Zhao
12. Split enzymes: Design principles and strategy
Shion A. Lim and James A. Wells
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