Foodborne Bacterial Pathogens, 1st ed. 2019
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1918

Language: English

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278 p. · 17.8x25.4 cm · Hardback

This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students,  and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens.


Part I: Bacterial Pathogen Detection and Quantification in Food 
1. Accelerated Sample Preparation for Fast Salmonella Detection in Poultry Products
Eduardo Ximenes, Seockmo Ku, Lori Hoagland, Michael R. Ladisch

2. Direct or DNA Extraction-free Amplification and Quantification of Foodborne Pathogens
Maggie R. Williams and Syed A. Hashsham

3. The Use of Multiplex Real Time PCR for the Simultaneous Detection of Food-borne Bacterial Pathogens
Alejandro Garrido-Maestu, David Tomás Fornés, and Marta Prado Rodríguez

4. Sequence Specific End Labelling of Oligonucleotides (SSELO)-based Microbial Detection
Tanja Kostic and Levente Bodrossy

5. Rapid on-site Detection and Quantification of Food-borne Pathogens Using Microfluidic Devices
Nobuyasu Yamaguchi

6. Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria
Matthew Dunne and Martin J. Loessner

7. EIS-based Biosensors in Foodborne Pathogen Detection with a Special Focus on Listeria monocytogenes
Palmiro Poltronieri, Elisabetta Primiceri, and Rajeswaran Radhakrishnan

Part II: Phenotypic and Metabolic Characterization of Foodborne Pathogens
8. Method to Study the Survival Abilities of Foodborne Bacterial Pathogens under Food Processing Conditions
Olivier Habimana

9. Viability Detection of Foodborne Bacterial Pathogens in Food Environment by PMA-qPCR and by Microscopy Observation 
Thomas Brauge, Graziella Midelet-Bourdin, and Christophe Soumet

10. Isolation of Bacterial RNA from Foods Inoculated with Pathogens
Deepti Tyagi, Autumn L. Kraft, and Teresa M. Bergholz

11. The use of Two-dimensional Electrophoresis to Explore Foodborne Bacterial Pathogens Responses to Gastrointestinal Stress
Denise Schrama and Maria Leonor Faleiro

12. Identification of Putative Biomarkers Specific to Food-borne Pathogens Using Metabolomics
Snehal R. Jadhav, Rohan M. Shah, Avinash V. Karpe, David J. Beale, Konstantinos A. Kouremenos, and Enzo A. Palombo

13. Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells 
Florence Dubois-Brissonnet

14. High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 
4D Stuctural Dynamic of Food-borne Pathogens 
Alexis Canette, Julien Deschamps, and Romain Briandet

15. Static Immersion and Injection Methods for Live Cell Imaging of Foodborne Pathogen Infections in Zebrafish Larvae 
Macarena A. Varas, Javiera Ortíz-Severín, Andrés E. Marcoleta, Carlos A. Santiviago , Miguel L. Allende, and Francisco P. Chávez 

16. Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection
Francisco P. Chávez and Andrea Calixto

Part III: NGS and Modeling Approaches

17. Utilizing the public GenomeTrakr Database for Foodborne Pathogen Traceback
Ruth E. Timme, Maria Sanchez Leon, and Marc W. Allard

18. The Establishment of a Standarized 16srDNA Library Preparation to Enable the Analysis of the Microbiome in Poultry Processing Using the Illumina MiSeq Platform
Kristina M. Feye and Steven C. Ricke

19. Exploring Foodborne Pathogen Ecology and Antimicrobial Resistance in the Light of Shotgun Metagenomics
Arnaud Bridier

20. Modelling Growth of Listeria and Lactic Acid Bacteria in Food Environments
P. Dalgaard and O. Mejlholm

21. Network Biology Approaches to Identify Molecular and Systems-level Differences between Salmonella Pathovars 
Marton Olbei, Robert A. Kingsley, Tamas Korcsmaros, and Padhmanand Sudhakar

Includes cutting-edge method and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts